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The in vivo endothelial cell translatome is highly heterogeneous across vascular beds (HUVEC RNAseq)

ABSTRACT: The in vivo endothelial cell translatome is highly heterogeneous across vascular beds (HUVEC RNAseq)

PROVIDER: PRJNA576634 | ENA |

REPOSITORIES: ENA

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The in vivo endothelial cell translatome is highly heterogeneous across vascular beds

Project description:The in vivo endothelial cell translatome is highly heterogeneous across vascular beds

| PRJNA576617 | ENA

The in vivo endothelial cell translatome is highly heterogeneous across vascular beds (HUVEC RNAseq)

Project description:The interspersed anatomic distribution of endothelial cells (ECs) and their dependence on microenvironmental cues have been major challenges in studying EC function and response to (patho)physiological stimuli in vivo. Combining EC-specific translating ribosome affinity purification (EC-TRAP) with RNA sequencing provides an accurate in vivo snapshot of tissue-specific EC expression profiles, and maintains a high degree of sensitivity to detect low abundant transcripts while limiting changes in gene expression profiles due to enzymatic tissue dissociation required to generate single cell suspensions for FACS sorting or single cell analysis. In vitro HUVEC data was obtained to ensure short-term cycloheximide treatment, as used for in vivo experiments, did not alter global gene expression.

2019-10-10 | GSE138628 | GEO

The in vivo endothelial cell translatome is highly heterogeneous across vascular beds (EC-TRAP RNAseq)

Project description:The in vivo endothelial cell translatome is highly heterogeneous across vascular beds (EC-TRAP RNAseq)

| PRJNA576635 | ENA

The in vivo endothelial cell translatome is highly heterogeneous across vascular beds (EC-TRAP RNAseq)

Project description:The interspersed anatomic distribution of endothelial cells (ECs) and their dependence on microenvironmental cues have been major challenges in studying EC function and response to (patho)physiological stimuli in vivo. Combining EC-specific translating ribosome affinity purification (EC-TRAP) with RNA sequencing provides an accurate in vivo snapshot of tissue-specific EC expression profiles, and maintains a high degree of sensitivity to detect low abundant transcripts while limiting changes in gene expression profiles due to enzymatic tissue dissociation required to generate single cell suspensions for FACS sorting or single cell analysis. In addition to demonstrating EC heterogeneity under physiologic conditions, the in vivo host response to lipopolysaccharide (LPS) revealed the induction of expression programs associated with a native defense response, with marked differences across vascular beds.

2019-10-10 | GSE138629 | GEO

The in vivo endothelial cell translatome is highly heterogeneous across vascular beds.

Project description: Not available

| S-EPMC6876253 | biostudies-literature

The in vivo endothelial cell translatome is highly heterogeneous across vascular beds

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-10-10 | GSE138630 | GEO

Molecular profiling of vascular beds in vivo by DISDIVO

Project description:We developed a new technique named as Differential Systemic Decellularization in vivo (DISDIVO) for the molecular profiling of vascular beds throughout the body. This method can by applied to murine models. Thus, the data provided here correspond to mice vascular beds obtained in vivo by DISDIVO.

2018-12-06 | PXD008794 | Pride

Implication of VSMC heterogeneity among arterial beds in vascular calcification

Project description:Transcriptome analysis of human primary smooth muscle cells from different arterial beds (abdominal and thoracic aorta, carotid and femoral and infrapopliteal arteries).

2018-01-31 | GSE84012 | GEO

Comparative Gene Expression Profiling of HUVEC and Ocular Vascular Endothelial Cells

Project description:To compare the gene expression profiles of unpassaged, proliferating HUVEC and human iris, retinal and choroidal microvascular endothelial cells. Gene expression profiling revealed significant differences between HUVEC and ocular microvascular endothelial cells suggesting that HUVE cells may not be a suitable surrogate when studying pathophysiological mechanisms of ocular disorders. There were significant differences in the gene expression of important cell signalling pathways in human retinal and choroidal ECs. These differences may be important in the mechanisms and treatment of choroidal and retinal neovascularisation.

2010-12-31 | GSE20986 | GEO

Regulation of highly expressed hCINAP on translatome

Project description:hCINAP is essential for human 18S rRNA processing and ribosome assembly. hCINAP is highly expressed in human cancers and promotes cancer cell growth. To connect the role of hCINAP in ribosome biogenesis and tumor growth, genome-wide polysome profiling was performed to detect the regulation of hCINAP on ribosome assembly and translation control. The results showed taht hihgly expressed hCINAP promotes ribosome biogenesis and selectively modulates cancer-associated translatome to promote malignancy. This result provides important insights into the molecular mechanism underlying the role of hCINAP in tumorigenesis.

2016-07-18 | GSE81469 | GEO

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