Project description:A 24h time-course array study was performed to analyse the different circadian phenotypes of LCL-H0 and HD-MY-Z cells, a cell line from B lymphoblastoid cells and a Hodgkin lymphoma cell line. Samples were taken every three hours for a period of 24h so that 9 time-points could be analysed for each cell line.
Project description:Interventions: For mucosal defect after ESD, only conventional clips are used and closed using Clip on Clip Closure Method.
Primary outcome(s): Evaluate whether mucosal defect after ESD is closed by Clip on Clip Closure Method using conventional clip.
Study Design: Single arm Non-randomized
Project description:Interstitial cells of Cajal (ICC) have important functions in regulation of motor activity in the gastrointestinal tract. In murine small intestine ICC are gathered in the region of the myenteric plexus (ICC-MY) and within the deep-muscular plexus near the submucosal surface of the circular muscle layer (ICC-DMP). These two classes of ICC have different physiological functions. ICC-MY are pacemaker cells and generate the slow wave electrical rhythmicity of gastrointestinal organs. ICC-DMP form synaptic connections with the varicose nerve terminals of enteric motor neurons and are involved in reception and transduction of motor neurotransmission. In the present study we used recently developed highly selective techniques to isolate the two classes of ICC from enzymatically dispersed intestinal muscles by fluorescence-activated cell sorting. Transcriptional expression of the two functional classes was investigated using DNA microarray analysis. Keywords: comparative transcriptional profiling
