Project description:Gene expression profile from brown adipose tissues of Prdm16 knockout and wile type mice. Prdm16 is a transcription factor that regulates the thermogenic gene program in brown and beige adipocytes. However, whether Prdm16 is required for the development or physiological function of brown adipose tissue (BAT) in vivo has been unclear. By analyzing mice that selectively lacked Prdm16 in the brown adipose lineage, we found that Prdm16 was dispensable for embryonic BAT development. Brown adipose tissues were collected from Prdm16 knockout and wiletype mice with 4 biological replicates per condition. Experiment was done in two separate batch for 6-week-old and 11-month-old. Extracted RNA was hybridized to Agilent two-color arrays.

2014-04-09 | E-GEOD-55080 | biostudies-arrayexpress

CRISPR/Cas9 inducible UCP1 knockout in brown adipose tissue of adult mice

Project description:Mice expressing Cas9 were administered AAV8-UCP1 sgRNA to the brown adipose tissue for inducible knockout. Mice were housed at 22ºC for two weeks and then were sacrificed. Brown adipose tissue (BAT) was harvested and RNAseq was performed to determine transcriptional changes stemming from inducible UCP1 knockout.

2021-07-01 | GSE176453 | GEO

RNA sequencing of brown adipose tissue in Letmd1 Knockout mice

Project description:We performed RNA sequencing on brown adipose tissue in wild-type and Letmd1 knockout mice to investigate the gene expression patterns in Letmd1 deficient mice.

2023-05-26 | GSE232223 | GEO

Taf7l Modulates Brown Adipose Tissue Formation

Project description:Brown adipose tissue (BAT) plays an essential role in metabolic homeostasis by dissipating energy via thermogenesis through uncoupling protein 1 (Ucp1). Previously, we reported that the TATA-binding protein Associated Factor 7L (Taf7l) is an important regulator of white adipose tissue (WAT) differentiation. Here, we show that Taf7l also serves as a molecular switch between brown fat and muscle lineages in vivo and in vitro. In adipose tissue, Taf7l containing TFIID complexes associate with PPAR to mediate DNA looping between distal enhancers and core promoter elements. Our findings suggest that presence of the tissue-specific Taf7l subunit in TFIID functions to promote long-range chromatin interactions during BAT lineage specification. mRNA-seq expression profiling wild type and Taf7l knockout interscapular brown adipose tissue (BAT)

2014-06-03 | E-GEOD-55797 | biostudies-arrayexpress

Analysis of the differences in gene expression between wild type and Gpr120 knockout brown adipose tissue

Project description:The goals were to investigate differences in gene expression between wild type and Gpr120 knockout mouse interscapular brown adipose tissue

2018-01-18 | GSE97145 | GEO

BATLAS: Deconvoluting brown adipose tissue

Project description:BATLAS: Deconvoluting brown adipose tissue

| PRJEB20634 | ENA

Atlas of Brown Adipose Tissue

Project description:Atlas of Brown Adipose Tissue

| PRJNA604636 | ENA

Brown adipose tissue monocytes support tissue expansion

Project description:Brown adipose tissue monocytes support tissue expansion

| PRJNA737132 | ENA