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Activated sludge cultivated in differed micro-sized silica particles

ABSTRACT: Micron-sized silica particles in wastewater changed the occurrence of extracellular polymeric substances (EPS) of sludge and influenced the anaerobic degradability of protein and polysaccharides

PROVIDER: PRJNA577327 | ENA |

REPOSITORIES: ENA

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RNAseq of human epithelial lung carcinoma cell line A549 exposed to ceria, silver or zinc ions, micron-sized particles or nanoparticles

Project description:The toxicity of silver and zinc oxide nanoparticles is hypothesised to be mediated by dissolved metal ions and cerium dioxide nanoparticles (CeO2 NPs) are hypothesised to induce toxicity specifically by oxidative stress dependant on their surface redox state. To test these hypotheses, RNAseq was applied to characterise the molecular responses of cells to metal nanoparticle and metal ion exposures. The human epithelial lung carcinoma cell line A549 was exposed to different CeO2 NPs with different surface charges, micron-sized and nano-sized silver particles and silver ions, micron-sized and nano-sized zinc oxide particles and zinc ions, or control conditions, for 1 hour, 6 hours and 24 hours. Concentrations were the lower of either EC20 or 128 micrograms/mL. Transcriptional responses were characterised by RNAseq transcriptomics using an Illumina HiSeq2500 .

2018-02-01 | E-MTAB-5734 | biostudies-arrayexpress

Vitamin C inhibits bacterial biofilms by affecting the synthesis of the extracellular polymeric matrix

Project description:Extracellular polymeric substances (EPS) produced by bacteria form a matrix for the complex three-dimensional architecture of biofilms. The EPS matrix of a biofilm is primarily composed of polysaccharides, proteins and extracellular DNA (eDNA). In addition to supporting the community structure, the EPS matrix protects bacterial biofilms from the environment. Specifically, the EPS matrix reduces the penetration of antimicrobial agents into the biofilm, thereby reducing their efficiency. Strategies for disrupting the formation of the EPS matrix can therefore lead to a more efficient use of antimicrobial agents. Here, we examine the biofilm-disrupting activity of vitamin C (sodium ascorbate). Quantitative proteomics analysis of vitamin C treated biofilms show that exposure to vitamin C inhibits quorum sensing and other regulatory mechanisms underpinning biofilm development. This results in inhibition of the EPS biosynthesis, and depletion of the polysaccharide component of the matrix. Once the EPS content is reduced beyond a critical point, bacterial cells get fully exposed to the medium, thereby experiencing a planktonic-like state. At this stage, the cells are more susceptible to killing, either by vitamin C-induced oxidative stress as reported here, or by other antibacterial compounds or treatments.

2017-12-19 | PXD007533 | Pride

activated sludge cultivated in differed micron-sized silica particles

Project description:Investigate the effects of silica particles on the anaerobic digestion performance and illustrate the influencing mechanism

| PRJNA554707 | ENA

Comparison of gene expresson between larger sized tetraploid and smaller sized diploid HCA2

Project description:In order to determine the factors that regulate senescence induction, we tried to identfy genes that are predominantly expressed in large sized senescent cells with tetraploid DNA. We sorted large or small sized HCA2 cells treated wth IR (10Gy) according to size and structure (FSC and SSC) by FACScan and analyzed global gene expression patterns.

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Proteomics on extracellular fractions of polyphosphate accumulating organisms from wastewater treatment plant

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2022-12-25 | PXD036522 | Pride

Transcription profiling of human lung epithelial cells (A549) treated with nano- and micro-sized silica and carbon particles to study toxic effects related to size and chemical origin

Project description:To screen for novel toxicological endpoints of nano-particles, we conducted an in vitro expression profiling study using human lung epithelial cells (A549). To identify effects that are specific to size or chemical origin, we compared gene expression of A549 cells treated with nano-sized and micro-sized particulates of two chemical origins (carbon and silica) to the control (untreated) cells.

2009-07-10 | E-TABM-679 | biostudies-arrayexpress

Comparison of gene expresson between larger sized tetraploid and smaller sized diploid HCA2

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Mechanism behind microalgal self-flocculation

Project description:Self-flocculation driven by gravity has shown as promising microalgal harvesting technology due to no requirement for additives and energies. However, the inherent unclear mechanism impedes its efficiency enhancement and commercial application. Current researches most only focus on the protein and carbohydrate content in extracellular polymeric substances (EPS), while further in-depth investigation is required on the detailed information of EPS components and their roles in self-flocculation.

2021-02-05 | PXD024007 | iProX

Julio EPS degradation

Project description:Different types of diatom-derived extracellular polymeric substances (EPS) drive changes in heterotrophic bacterial communities from intertidal sediments

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