Similar Datasets
Project description:The search for more effective methods to alleviate the negative effects of abiotic stresses in plants has motivated the experimentation of new technologies, namely nanotechnologies. It is in this context that the use of formulations containing hybrid silicon (Si) nanoparticles (NPs) and acting as delivery systems of the flavonoid quercetin was here investigated. These formulations, previously referred to as phyto-couriers, proved their efficacy in protecting textile hemp against salinity. We here broaden their application spectrum by studying the effects on an important crop model, tomato (Solanum lycopersicum cv. Micro-Tom). Two phyto-courier formulations, labelled GS1 and GS3, functionalized with 25 mg of quercetin and differing in the presence of trehalose were applied to salt-stressed plants (200 mM NaCl) by foliar spraying. Microscopy showed, under stress, a preservation of the palisade mesophyll cell structure in plants treated with the formulations. A trend towards decreased expression of some stress-responsive genes was observed in the leaves treated with the phyto-couriers. Shotgun proteomics confirmed the protective effects and the nano-biostimulant nature of the formulations, since several proteins involved in cytoprotection against oxidative stress were more abundant in control leaves treated with the pyto-courier. This was especially evident for the trehalose-containing GS3 formulation. Some proteins involved in chromatin remodelling were also more abundant in control leaves treated with GS3. Overall, the formulations showed promising results to enhance abiotic stress tolerance in a model crop through the mitigation of stress symptoms. The results presented are a proof-of-concept for the use of the phyto-courier nanotechnology in horticultural applications
2023-03-06 | PXD035460 | Pride
Project description:Phyto melanocephala, genomic and transcriptomic data
| PRJEB51914 | ENA
Project description:Phyto melanocephala genome assembly, idPhyMeln1, alternate haplotype
| PRJEB52669 | ENA
Project description:Using quantitative proteomics we identified group of synaptic genes with decreased protein synthesis during homeostatic plasticity. To obtain further information about their mRNA levels/sequences (3’UTR) we performed polyA RNAseq. Using EISA analysis of Ribominus RNAseq dataset we could further differentiate between transcriptional respective post-transcriptional dependent alterations in mRNA levels. In addition Ribominus RNAseq from cell body and processes (dendrites, axons) RNA samples showed us local changes in mRNA levels during homeostatic plasticity. At the end, small RNAseq helped us to identify miRNAs that are increased during homeostatic plasticity and might regulate downregulated genes.
2017-04-05 | PXD004150 | Pride
Project description:Cascading effect of grazer signalling molecules on marine phyto- and bacterio-plankton
| PRJEB25550 | ENA
Project description:Ba/F3 cells were transformed after transfection with CRISPR/CAS9 + gRNA vs target gene. Oligoclonal cell population was flow sorted into single cells and processes for RNAseq.
2014-12-09 | E-ERAD-298 | biostudies-arrayexpress