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RNA-seq of NIH3T3 cells treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

ABSTRACT: RNA-seq of NIH3T3 cells treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

PROVIDER: PRJNA591160 | ENA |

REPOSITORIES: ENA

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RNA-seq of NIH3T3 cells treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

Project description:To identify gene expression changes associated with treatment of EVs from MDA-MB-231 [231] and MCF10A [10A] cells) in NIH3T3 cells, we analyzed RNA isolated from PBS- or EV-treated NIH3T3 cells. Gene expression in NIH3T3 cells treated with EV from MDA-MB-231 cells was compared to cells treated with PBS or EV from MCF10A cells, both of which served as controls in this experiment.

2020-05-01 | GSE140852 | GEO

RNA-seq of liver tissue from mice treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

Project description:To identify gene expression changes in liver associated with circulating EV that carry high levels of miR-122 (from MDA-MB-231 and MCF10A/miR-122 cells), we analyzed RNA isolated from the liver of PBS- or EV-treated female NOD scid gamma (NSG) mice. Mice had received tail-vein injected EV (or PBS) twice a week for 5 weeks (~10 ug EV per injection). Gene expression in liver from mice treated with EV from MDA-MB-231 or MCF10A/miR-122 cells was compared to cells treated with PBS or EV from MCF10A cells, both of which served as controls in this experiment.

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RNA-seq of cancer-associated fibroblasts (CAF) treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

Project description:To identify gene expression changes associated with treatment of EV that carry high levels of miR-105 (from MDA-MB-231 and MCF10A/miR-105 cells) in human breast tumor derived CAF, we analyzed RNA isolated from PBS- or EV-treated CAF. Gene expression in CAF treated with EV from MDA-MB-231 or MCF10A/miR-105 cells was compared to cells treated with PBS or EV from MCF10A cells, both of which served as controls in this experiment.

2018-02-11 | GSE106503 | GEO

RNA-seq of liver tissue from mice treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

Project description:RNA-seq of liver tissue from mice treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

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RNA-seq of cancer-associated fibroblasts (CAF) treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

Project description:RNA-seq of cancer-associated fibroblasts (CAF) treated with PBS or extracellular vesicles (EV) from MCF10A or MDA-MB-231 cells

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Murine NIH3T3 Cells: Wild vs. BRAFV600E Transfected

Project description:Transcriptional profiling of mouse NIH3T3 cells comparing control NIH3T3 cells transfected with a pEFm6-BRAF with cells transfected with pEFm6-BRAFV600E. Goal was to determine the effects of BRAFV600E gene transfection on global mouse NIH3T3 cells gene expression.

2017-01-01 | GSE90589 | GEO

RNA-seq of MCF10A transduced with an inducible lentiviral MYB expression system

Project description:The non-tumourigenic breast cell line MCF10A was transduced using pINDUCER21-MYB vector to express MYB upon addition of doxyclyclin (DOX), and compared to an empty vector (EV) control (pINDUCER21 (ORF-EG)) with and without the addition of DOX

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Circadian rhythm in NIH3T3 cells

Project description:Identification of cyclical expressed coding and non-coding genes during the circadian rhythm in NIH3T3 cells. NIH3T3 cells were synchronized for their circadian rhythm and RNA sequencing were performed at several time points along the rhythm. This data was used to identify cyclical expressed genes as well as long intergenic non-coding RNAs.

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Mus musculus breed:NIH3T3 cells

Project description:NIH3T3 RNA-Seq after stress

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Effect of acute HGF-induced cMET activation in MCF10A cells in vitro

Project description:cMET is a well known oncogene whose activation is widely implicated in tumorigenesis and metastasis. To investigate the effects of acute activation of cMET signaling in the mammary epithelial cells, we stimulated MCF10A cells with increasing doses of HGF and analyzed the transcriptional alteration between PBS and HGF treated cells.

2020-02-23 | GSE52096 | GEO

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