Project description:RNA-seq profile of transgenic hindbrains expressing heat-shock induced constitutively activated fgfR1 (Tg(hsp70:ca-fgfr1)) and compared to heat-shocked counterparts Method: 22hpf embryos were heat-shocked for 30min at 38.5°C and then incubated for 2hr at 28.5°C . After hindbrains were microdissected and the RNA was extracted. fgfR1 overexpression was checked by qPCR and samples were selected for sequencing
Project description:Purpose: The goal of this study is to understand the progressive patterning of neurogenesis of the developing zebrafish hindbrain. 16hpf, 24hpf and 44hpf zebrafish hindbrains were used for single-cell RNA-sequencing with the aim to uncover hindbrain development. Methods: 40 microdissected hindbrains per each stage were dissociated at loaded into the 10x Genomics Chromium Platform, and sequenced using Illumina HiSeq 4000. Conclusions: Our study constitute a resource of hindbrain gene expression during development. We have identified transcriptional programs involved in: rhombomere segmental identity, dorso-ventral patterning, boundary and centre progenitor cells and temporal regulation of neurogenesis.
Project description:identification of differentially expressed genes in gas6 homozygous mutant hindbrain when compared to wildtype hindbrain in zebrafish
Project description:The molecular logic that specifies and assembles closely-related subtypes of neurons into functional sensorimotor circuits remains unclear. The goal of this study was to characterize the molecular profiles of hindbrain vestibular neurons in the larval zebrafish to identify candidate molecular programs that specify their subtype fate, topography, and circuit assembly. We used single-cell RNA sequencing to generate a comprehensive atlas of hindbrain vestibular neurons and fluorecent in situ hybridization to annotate profiled neurons. Our dataset serves as a reference for evaluating developmental changes in molecular profiles following perturbations and identifies new candidate molecular solutions that assemble closely-related subtypes into functional circuits.