Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

LTi-like cells in the gut, mLN and spleen

ABSTRACT: LTi-like cells in the gut, mLN and spleen

PROVIDER: PRJNA594368 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

LTi-like cells in the gut, mLN and spleen

Project description:We plan to describe different characters of LTi-like cells from distinct tissues. Also, we further compare the LTi-like cells from both Id2fl/fl and RorccreId2fl/fl mLN and gut.

2020-07-09 | GSE141689 | GEO

RNA-seq analysis of spleen and mesenteric lymph node (mLN) hematopoietic cells

Project description:To search for factors that affect naive CD4+ T (TN) cell phenotypes in spleen, we performed RNA-seq analysis to compare mRNA expression between freshly-prepared spleen and mLN hematopoietic cells.

2024-03-01 | GSE215774 | GEO

Comparison of mRNA expression between spleen and mesenteric lymph node (mLN) naive CD4+ T (TN) cells

Project description:To analyze difference between spleen and mLN TN cells, RNA-seq analysis was performed on TN cells freshly prepared from each organ.

2024-03-01 | GSE215775 | GEO

MLN

Project description:MLN

| PRJEB7634 | ENA

In-vitro response pattern of mesenteric lymph node (MLN) cells to specific gut commensal microbes

Project description:Gut microbiota plays a significant role in shaping gut and systematic immune and huge strain- and species- dependent differences exit. Using in-vitro MLN co-culture models, we want to initially uncover these differences. MLN cells were isolated from C57BL/6 mice and co-cultured with bacterial cells with or without anti-CD3 and anti-CD28 antibodies for three days. After that, IL-10 in cell supernatant was measured and RNA was extracted using Trizol reagent. RNA-seq was performed on Illumina Hiseq X ten. Raw sequenced data were filtered by fastp and clean data were mapped to reference genome (Mus_musculus, GRCm39). Data processing adopted featurecounts in subread, abundance_estimates_to_matirx.pl in Trinity, DEseq and ClusterProfiler. We found that all bacterial species activated the innate immunity response. However, only Akkermansia muciniphila and Clostridium butyricum had cognate T cell antigen and activated adaptive immunity responses even without stimulus.

2022-11-21 | GSE189176 | GEO

Gene expression profile in CD11c+ splenic dendritic cells and CD11c+CD103+ dendritic cells from mesenteric lymph nodes (MLN) of mice with DC-specific targeting of TGFBR2

Project description:Transcriptome analysis of dentritic cells from the spleen (CD11c+) or MLN (CD11c+CD103+) of mice with DC-specific deletion of TGFBR2 gene, or their control littermates. TRGFR2 gene was deleted in dendritic cells using Cre/lox approach. Mice with this deletion develop spontaneous multi-organ autoimmune inflammation and die by 15 weeks of age. Splenic CD11c+ Dc were isolated by magetic cell sorting. MLN CD11c+CD103+ DC were flow sorted. RNA was isolated using RNAqueous-Micro kit (Ambion) and analyzed using Affymetrix Mouse Exon 1.0 ST Array

2012-07-25 | E-GEOD-39651 | biostudies-arrayexpress

Gene expression profile in CD11c+ splenic dendritic cells and CD11c+CD103+ dendritic cells from mesenteric lymph nodes (MLN) of mice with DC-specific targeting of TGFBR2

2012-07-26 | GSE39651 | GEO

Transcriptomics of MLN-expressing cells in human duodenum organoids

Project description:We purified two populations of human duodenal MLN-expressing enteroendocrine cells (Venus+ and Venus-) from mature differentiated hMLN-Venus organoids by FACS and identified transcripts enriched in endocrine cell lineages.

2021-12-21 | GSE176552 | GEO

Transcriptomics of MLN-expressing cells in human duodenum organoids

Project description:Transcriptomics of MLN-expressing cells in human duodenum organoids

| PRJNA736695 | ENA

The signaling pathway involved in wild type and 2D2-gut CD4+ T cell development

Project description:To find the signalling pathway involved in WT/2D2 CD4+ gut T cell (CD4+ intraepithelial lymphocytes, CD4+IEL) differentiation, particulary to determine whether aryl hydrocarbon receptor (AHR) pathway is involved. Gene expression was analyzed ex vivo in CD4+ T cells that were sorted from Wild type-Gut / 2D2 mouse-Gut / Wild type-Spleen / 2D2 mouse-Spleen (N=3 per group). The genes highly expressed in WT/2D2 Gut compared to WT/2D2 Spleen were selected by k-means clustering. AHR pathway related genes (IPA software) were further selected.

2016-04-01 | E-GEOD-79748 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data