Ontology highlight
ABSTRACT:
PROVIDER: PRJNA595342 | ENA |
REPOSITORIES: ENA
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Project description:Prymnesium parvum is regarded as one of the most notorious harmful algal bloom (HAB) species worldwide. In recent years, it has frequently formed toxic blooms in coastal and brackish waters of America, Europe, Australia, Africa and Asia, causing large-scale mortalities of wild and cultured fish and other gill-breathing animals. In the last decade, blooms of P. parvum have expanded to inland fresh waters in the USA, presumably due to changes in environmental conditions. The aim of the experiment was to establish the gill transcriptomic responses to P. parvum in rainbow trout. We used 2 different concentrations of P. parvum and identified fish with low and moderate responses to the algae. Based on the dose of and the fish response, fish were classified into 4 groups with high exposure/moderate response (HM), high exposure/low response (HL), low exposure/low response (LL) and control group (C) with no exposure/no response. Gene expression profiling of the gill tissue was performed using a microarray platform developed and validated for rainbow trout.
2021-11-10 | E-MTAB-10541 | biostudies-arrayexpress
Project description:The inhibition of Harmful algal blooms (HABs) or red tides
| PRJNA706502 | ENA
Project description:Tursiops truncatus RAD sequencing for study of adaptation to harmful algal blooms
| PRJNA241352 | ENA
Project description:Nanoscale zero valent iron (nZVI) is used to remediate aquifers polluted by organochlorines or heavy metals has been also considered for elimination of harmful algal blooms. Highly reactive nZVI then affects microorganisms in the application area. To date, various nZVI toxicity endpoints have been studied on different organisms. However, the underlying mechanistic related to iron defense pathways have not been explained sufficiently. Here we aim to describe the physiological and transcriptomic response of the microalga, Raphidocelis subcapitata ATCC 22662, to 100 mg/L of non-reactive nFe3O4, and reactive nZVI. The combined effect of shading by nanoparticles and release of Fe2+ from nZVI posed a stronger inhibition leading to deformed cells and cytosol leakage in 15% of cells. Transcriptomic analysis confirmed the stronger physiological effect of nZVI (7,380 differentially expressed genes [DEGs]) than nFe3O4 (4,601 DEGs) after 1 h. nZVI (but not nFe3O4) caused increased DNA repair and replication, while deactivated carbohydrate-energy metabolisms, mitochondria signaling, and transmembrane ion transport. The defense response of algal cells was immediate to successfully face oxidative stress.
2023-06-06 | GSE234088 | GEO
Project description:Harmful algal blooms are induced largely by nutrient enrichment common in warm waters. An increasingly frequent phenomenon is the “red tide”: blooms of dinoflagellate microalgae that accumulate toxins lethal to other organisms in high doses. Here, we present the de novo assembled genome (~4.75 Gbp) of Prorocentrum cordatum, a globally abundant, bloom-forming dinoflagellate, and the associated transcriptome, proteome, and metabolome data from axenic cultures to elucidate the microalgal molecular responses to heat stress. We discovered, in a high-G+C genome with long introns and extensive genetic duplication, a complementary mechanism between RNA editing and exon usage that regulates dynamic expression and functional diversity of genes and proteins, and metabolic profiles that reflect reduced capacities in photosynthesis, central metabolism, and protein synthesis. These results based on multi-omics evidence demonstrate the genomic hallmark of a bloom-forming dinoflagellate, and how the complex gene structures combined with multi-level transcriptional regulation underpin concerted heat-stress responses.
2023-10-18 | PXD046193 | Pride
Project description:Iron limitation effects on nitrogen-fixing organisms with implications for cyanobacterial harmful algal blooms
| PRJNA417492 | ENA
Project description:Harmful algal blooms (HABs) produce potent neurotoxins that threaten human health. Early life exposure to low levels of the HAB toxin domoic acid (DomA) can produce long-lasting behavioral deficits, but the mechanisms involved are unknown. Using the zebrafish model, we investigated the developmental window of susceptibility to low doses of DomA and examined cellular and molecular targets. Larvae exposed to DomA (0.09 ng) at 2 days post-fertilization (dpf), but not at 1 or 4 dpf, showed deficits in startle behavior that included reduced responsiveness and altered kinematics. Similarly, myelination in the spinal cord was disorganized after exposure at 2 dpf but not 1 or 4 dpf. Time-lapse imaging revealed disruption of the initial stages of myelination. DomA down-regulated genes required for maintaining myelin structure and the axonal cytoskeleton. These results identify a developmental window of susceptibility to DomA-induced behavioral deficits involving altered gene expression and disrupted myelin structure and establish a zebrafish model for investigating the underlying mechanisms.
2019-12-06 | GSE140045 | GEO