RNA-seq of subcutaneous and visceral adipose tissue from sheep prenatally exposed to BPA
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ABSTRACT:
PROVIDER: PRJNA596241 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Subcutaneous and visceral adipose tissue from sheep prenatally exposed to BPA
2020-04-30 | GSE142222 | GEO
Project description:Developmental programming is the concept that environmental factors during fetal growth can alter development, metabolism and physiology of an organism, thus diseases can be programmed by intrauterine influences. The fetal origin of health and disease hypothesis has received strong support from experimental studies in animals including livestock species. Using a sheep model, it has been shown that developmental changes of ewe offspring may be susceptible to maternal pregnancy bisphenol A (BPA) treatment. For example, prenatally BPA treated female offspring tended to have reduced birth weight. In this study we sought to identify gene expression differences in ewe offspring as a result of maternal pregnancy BPA treatment on a control or high-caloried diet. Samples of heart tissues were collected during the second breeding season, allowing us to examine gene expression changes in ewes prenatally treated with BPA and maturing under the different diets.
2017-01-29 | GSE77418 | GEO
Project description:Obesity is characterized by the excess of body fat leading to impaired health. Abdominal fat is particularly harmful and is associated with cardiovascular and metabolic diseases and cancer. In contrast, subcutaneous fat is generally considered less detrimental. The mechanisms that establish the cellular characteristics of these distinct fat types in humans are not fully understood. Here, we explored whether differences of their gene regulatory mechanisms can be investigated in vitro. For this purpose, we in vitro differentiated human visceral and subcutaneous pre-adipocytes into mature adipocytes and obtained their gene expression profiles and genome-wide H3K4me3, H3K9me3 and H3K27ac patterns. Subsequently, we compared those data with public gene expression data from visceral and subcutaneous fat tissues. We found that the in vitro differentiated adipocytes show significant differences in their transcriptional landscapes, which correlate with biological pathways that are characteristic for visceral and subcutaneous fat tissues, respectively. Unexpectedly, visceral adipocyte enhancers are rich on motifs for transcription factors involved in the Hippo-YAP pathway, cell growth and inflammation, which are not typically associated with adipocyte function. In contrast, enhancers of subcutaneous adipocytes show enrichment of motifs for common adipogenic transcription factors, such as C/EBP, NFI and PPARgamma, implicating substantially disparate gene regulatory networks in visceral and subcutaneous adipocytes. Consistent with the role in obesity, predominantly the histone modification pattern of visceral adipocytes is linked to obesity-associated diseases. Thus, this work suggests that the properties of visceral and subcutaneous fat tissues can be studied in vitro and provides preliminary insights into their gene regulatory processes.
2018-06-30 | GSE102638 | GEO
Project description:Adipose tissue (AT) distribution is an important determinant of cardiometabolic health. Increased visceral adiposity has been associated with a higher risk of obesity-related diseases. An important step in deciphering the molecular complexity of subcutaneous AT (SAT) and visceral AT (VAT) is to elucidate the molecular composition of the principal AT cell type, adipocytes. We present a comprehensive protein expression profile of abdominal subcutaneous (SA) and omental visceral adipocytes (VA). We isolated adipocytes from paired AT biopsies obtained during bariatric surgery of 19 morbidly obese women (BMI > 30 kg/m2) and performed state-of-the-art mass spectrometry to investigate their proteome profiles. We identified 3,686 proteins groups and found 1,140 differentially expressed proteins (adj. p-value < 0.05), of which 576 proteins were upregulated in SA and 564 in VA samples. In addition to providing a global protein profile of abdominal SA and omental VA, we also present the most differentially expressed pathways and processes distinguishing SA from VA. We show that SA are significantly more active in processes linked to vesicular transport and secretion, and also to increased lipid metabolism activity. Conversely, the expression of proteins involved in the mitochondrial energy metabolism and translational or biosynthetic activity is higher in VA. We also performed prediction analyses of differentially expressed putative secreted proteins, which revealed a significantly higher number of potentially secreted proteins in SA compared to VA. Our analysis represents a valuable resource of protein expression profiles in abdominal SA and omental VA, highlighting key differences in their role in obesity. Additionally, we predict possible protein targets among secreted proteins, which may be utilized for the further investigation of the role of different AT depots in obesity using peripheral blood.
2021-11-04 | PXD024734 | Pride
Project description:Gestational BPA exposure in sheep induces metabolic phenotype in sheep characterized by peripheral insulin resistance and increased adipocyte size. Because insulin sensitivity can be regulated by various agents including free fatty acids (FFA), we hypothesize that gestational BPA exposure alters circulating FFA inducing dyslipidemia, a marker of metabolic disorder. Because saturated FFA is associated with insulin resistance, determination of the FFA profile in these species aid in understanding the underlying mechanism. Additionally, because of the non-monotonic nature of responses to BPA exposure dose response studies are also needed. This study will therefore assess plasma lipid profile in sheep exposed to three different doses of BPA prenatally and compared with untreated control animals
2016-07-05 | ST000686 | MetabolomicsWorkbench
Project description:To investigate the proteomic profiles of paired subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) samples, as well as their correlations with clinical traits in severely obese patients, and to identify potential serum protein markers associated with tissue expression or metabolic states.
2023-10-16 | PXD041721 | Pride
Project description:Distinct characteristics of adipose tissue at different localization of human body has shown greater significance in development of metabolic disorders. Visceral adipose tissue in particular is known to be associated with obesity related metabolic complications that include type II diabetes. In this experiment, we attempt to profile transcriptome signatures of adipocyte, stromal vascular fraction (SVF) and adipose tissue from subcutaneous and visceral adipose tissue from obese individuals.
2019-11-04 | GSE135776 | GEO
Project description:Transcriptomes of visceral and subcutaneous adipose tissue from colorectal cancer patients were profiled to investigate differences between depots. Samples were collected at time-point of primary surgery.
2019-06-22 | E-MTAB-8013 | biostudies-arrayexpress
Project description:Adipose tissue is a primary regulator of energy balance and metabolism. The distribution of adipose tissue depots is of clinical interest because the accumulation of upper-body subcutaneous (ASAT) and visceral adipose tissue (VAT) is associated with cardiometabolic diseases, whereas lower-body gluteal-femoral adipose tissue (GFAT) appears to be protective. There is heterogeneity in the morphological and metabolic traits of adipocytes obtained from different regions of the body, but detailed knowledge of the constituent proteins in each depot is lacking. Here, we determined the human adipocyte proteome from ASAT, VAT and GFAT using high-resolution SWATH mass spectrometry proteomics.
2021-04-19 | PXD018314 | Pride
Project description:The aim of this study was to undertake an in-depth and comparative study of the protein expression patterns of subcutaneous and visceral adipose tissues in goats, combined to an mRNA expression study of proteins involved in immune and inflammatory response. Samples were obtained from four healthy goat-kids, Alpine breed, naturally reared by their mothers. Animals were slaughtered at the age of 30 days, during routinely slaughtering procedures, and four different adipose tissues were collected from each animal. Subcutaneous fat was taken from sternum and base of the tail; visceral fat was taken from perirenal and omental depots. The four adipose tissues deposits were selected due to their frequent use in experimental studies on fat tissue. Liver samples were also collected, and used as reference samples. Tissue samples were snap frozen in liquid nitrogen and stored at –80 degrees C until analysis. The 20 runs included in this dataset are from the 4 animals x 5 tissues (sternum, base of the tail, perirenal area, omentum and liver). A 2-D label free LC-MS/MS approach followed by cluster analysis was used for comparing the subcutaneous and visceral fat tissue proteomes. Each sample was analysed in ~12 SCX fractions which correspond to the WIFF files included in each RAW_GOAT0xx.zip files.
2015-07-17 | PXD000564 | Pride