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Physiological and transcriptomic analyses reveal sulfur is essential for cadmium detoxification and accumulation in poplar leaves

ABSTRACT: Physiological and transcriptomic analyses reveal sulfur is essential for cadmium detoxification and accumulation in poplar leaves

PROVIDER: PRJNA597470 | ENA |

REPOSITORIES: ENA

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Physiological and transcriptomic analyses reveal sulfur is essential for cadmium detoxification and accumulation in poplar leaves

Project description:Although poplars are good candidates for the phytoremediation of cadmium (Cd)-polluted soils and sulfur is a key player in modulating Cd detoxification and accumulation in plants, the physiological and molecular mechanisms underlying sulfur-mediated Cd accumulation remain largely unknown. To investigate physiological and transcriptomic regulation mechanisms of poplars in response to Cd exposure and different sulphate (S) supply levels, Populus deltoides saplings were exposed to either 0 or 50 µM Cd together with one of low, moderate and high S levels. Cd was accumulated in the leaves of Cd-exposed poplars under moderate S condition, and its accumulation is inhibited by low S but tended to increase by high S. Sulfur nutrition became deficient in Cd-treated leaves, and it was aggravated by low S but improved by high S. Concentrations of cysteine and glutathione were increased in Cd-treated leaves, and the Cd-induced increases were limited by low S but stimulated by high S. In line with the physiological changes, a number of mRNAs and microRNAs (miRNAs) involved in Cd accumulation and sulfur assimilation were identified and the miRNAs-mRNAs networks were dissected. In the networks, miR395 and miR399 members were identified as hub miRNAs and their targets were ATP sulfurylase 3 (ATPS3), and phosphate 2 (PHO2) and beta galactosidase 1 (BGAL1), respectively. These results suggest that Cd-induced changes in Cd accumulation and sulfur assimilation are exacerbated by low S but alleviated by high S supply, and that miRNAs-mRNAs regulatory networks play pivotal roles in sulfur-mediated Cd detoxification and accumulation in poplars.

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Transcriptome profiling of Hansenula polymorpha in response to sulfur starvation and cadmium stress

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Transcriptome profiling of Hansenula polymorpha in response to sulfur starvation and cadmium stress

Project description:Transcriptome profiling analysis of the Hansenula polymorpha MET4 gene deletion strain have been carried out to obtain comprehensive information on the HpMet4p-mediated regulatory networks in association with the cadmium (Cd) detoxification and sulfur regulation in H. polymorpha. Total RNA samples were collected from H. polymorpha wild type, and HpMET4 deletion strain, under sulfur starvation or Cd (0.6 mM) stress conditions. The differential fluorescence intensities of each RNA sample were measured after labeling with Cy3 or Cy5. For all analyses, we performed dye swapping experiments to avoid dye bias. Thus, four intensity values were generated for each ORF and averaged for analysis.

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Drivers of metallothionein-mediated detoxification of cadmium in C.elegans

Project description:Cadmium is toxic and intricate pathways linked to metallothioneins (MT) drive the detoxification process. Whist the mechanisms are well understood in mammalian systems, detailed knowledge is still elusive in invertebrates (which notably differ to mammalian systems). The model nematode Caenorhabditis elegans is ideally suited for assessing metallothionein mediates detoxification in invertebrates as is relatively short-lived, can be easily exposed and its genome is fully sequenced and widely annotated. The aim of the experiment was to identify new MT mediated target genes involved in Cd toxicosis.

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Threonine dehydratase couples L-cysteine metabolism to cadmium resistance in Pseudomonas stutzeri 273 through cadmium sulfide nanoparticle biomineralization

Project description:Cysteine is an amino acid containing sulfhydryl (-SH) group which is the basis of cysteine involved in sulfur metabolism and heavy metal detoxification in microorganisms. Here, we demonstrate that adding L-cysteine significantly improves the cadmium resistance and removal ability of Pseudomonas stutzeri 273. Threonine dehydratase (TSD) connects L-cysteine metabolism and cadmium resistance through CdS nanoparticle biomineralization with the ability of catalyzing L-cysteine desulfuration and H2S generation in P. stutzeri 273. Gene knockout of TSD in P. stutzeri 273 results in the decrease of L-cysteine and cadmium resistance, decline of H2S generation, and reduction of CdS biosynthesis ability.

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The ferruginous hemeprotein HhuH facilitates the detoxification of cadmium and chromium in Stenotrophomonas sp. SY1

Project description:Stenotrophomonas sp. SY1 showed the highly efficient detoxification to cadmium and chromium. Thus, the purpose of this project is to clarify the mechanism of detoxification to cadmium and chromium in strain SY1.

2024-02-29 | PXD050232 | iProX

Transcription profiling of Arabidopsis treated with of two cadmium concentrations on leaves and roots at three different times

Project description:Dynamic of the Arabidopsis thaliana transcriptome following a cadmium exposition.<br> The goal of the project is to developp a global approach without a priori in order to identify the key players involved in response to cadmium: signalisation and mechanisms of detoxification in the model plant Arabidopsis thaliana. An originality of this project is to investigate the response of this organism by analyzing separately leaves and roots. This analysis will be performed in response to sub-toxic and toxic levels at different times. <br> Effects of two cadmium concentrations on leaves and roots at three different times.

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Project description:Purpose: In order to explore the detoxification mechanism of nitrogen against cadmium stress in plants Methods:Cultivation and treatment for four months of P.yunnanensis seedlings by soil culture, in triplicate, qRT–PCR validation was performed using TaqMan and SYBR Green assays Results:Exogenous nitrogen induced the expression of resistance genes Conclusions: Our study represents that exogenous nitrogen addition under Cd stress induced more differential expressed TFs.

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Transcriptome changes in pea leaves with sulfur deficency/sufficiency during reproductive phase

Project description:transcriptome changes in pea leaves with sulfur deficency/sufficiency during reproductive phase.-Characterization of transcriptome changes in leaves of wild-type and PsSultr4 mutant lines (for a sulfur transporter) subjected or not to sulfur deficiency during the reproductive phase 4plex_pea_2014_01 - transcriptome changes in pea leaves with sulfur deficency/sufficiency during reproductive phase. - Role of sulfur and of the sulfate store in leaf metabolism. - Comparison of: 1- The leaf transcriptome of pea subjected or not to sulfur deficiency during the reproductive phase (S+ versus S –) 2- The leaf transcriptome of wild-type and mutant lines for a sulfur transporter (two TILLING alleles) grown under sulfur sufficient conditions : WT1/Mut1 S+ et WT2/Mut2 S+ 3- The leaf transcriptome of wild-type and mutant lines for a sulfur transporter (two TILLING alleles) grown under sulfur deficient conditions : WT1/Mut1 S+ et WT2/Mut2 S+

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