Project description:To determine the molecular basis for the dual oxidase in circulation in duox mutant zebrafish, we performed high-throughput sequencing at 4 dpf. Results revealed 375 up-regulated genes and 360 down-regulated genes in the duox mutant zebrafish compared with wild-type zebrafish. We found that some mitochondrial genes are down-regulated in duox mutant zebrafish, which was reconfirmed with qRT-PCR.
Project description:We generated a 16 nucleotide deletion mutant in the zebrafish fbln1 gene and we compared the whole larvae transcriptome to wt siblings in 10dpf larvae.
Project description:Gene expression profiles in lri12 mutant zebrafish larvae at 5 dpf based on RNAseq data. This dataset was also used to clone the mutation.
Project description:The zebrafish Cxcr3.2 is a functional homolog of the human chemokine receptor CXCR3. Zebrafish macrophages lacking this receptor have impaired motility and a rounded shape compared to their wildtype counterparts. To investigate the effects of cxcr3.2 mutation on the transcriptional profile of macrophages, we sorted macrophages from zebrafish larvae lacking a functional cxcr3.2 and compared their transcriptome to that of macrophages from wildtype larvae. Mutant and wildtype macrophages could be clearly distinguished based on the overall differential expression profiles. Classification of genes by compartment showed that peroxisomal, lysosomal and Golgi-related genes were most frequently up-regulated. Moreover, lysosomal and Golgi-related terms were significantly differentially represented in Gene Ontology and KEGG enrichment analysis. Of note, several lysosomal markers (including acidic hydrolases and voltage ATPases) were consistently upregulated in cxcr3.2 mutant macrophages, indicating that cxcr3.2-mediated chemokine signaling is tightly connected to the regulation of lysosomal function.
Project description:The purpose of the experiment was to identify the effects of pheta1 and pheta2 on the expression of genes associated with chondrogenesis and osteogenesis