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Myeloma-modified 3T3-L1 adipocytes exhibit aberrant gene expression profiles [experiment 2]

ABSTRACT: Myeloma-modified 3T3-L1 adipocytes exhibit aberrant gene expression profiles [experiment 2]

PROVIDER: PRJNA599433 | ENA |

REPOSITORIES: ENA

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Myeloma-modified 3T3-L1 adipocytes exhibit aberrant gene expression profiles

Project description:Myeloma-modified 3T3-L1 adipocytes exhibit aberrant gene expression profiles

| PRJNA599429 | ENA

Myeloma-modified 3T3-L1 adipocytes exhibit aberrant gene expression profiles [experiment 1]

Project description:Myeloma-modified 3T3-L1 adipocytes exhibit aberrant gene expression profiles [experiment 1]

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Myeloma-modified 3T3-L1 adipocytes exhibit aberrant gene expression profiles [experiment 1]

Project description:Herein we compare mouse 3T3-L1 adipocyte gene expression after 7 days of adipogenic differentiation with and without exposure to MM.1S myeloma cells. Microarray analysis was used to compare adipogenic gene expression either following exposure to MM.1S cells prior to differentiation (48h) or after differentiation (72h).

2021-03-10 | GSE143267 | GEO

Myeloma-modified 3T3-L1 adipocytes exhibit aberrant gene expression profiles [experiment 2]

2020-11-01 | GSE143268 | GEO

Myeloma-modified 3T3-L1 adipocytes exhibit aberrant gene expression profiles

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Myeloma-modified adipocytes exhibit a senescent-associated secretory phenotype

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RNA-sequencing of 3T3-L1 preadipocytes and adipocytes

Project description:RNA-sequencing of 3T3-L1 preadipocytes and adipocytes

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Glucocorticoid target genes in 3T3-L1 adipocytes

Project description:Analysis of 3T3-L1 adipocytes treeated with dexamethasone (Dex) for 6 hours. Dex is a synthetic glucorticoid (GC) receptor agonist. Results provide insight into the effect of glucocorticoids on adipocytes.

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Gene expression signatures in IRF7 overexpressed 3T3-L1 adipocytes

Project description:To identify the genes that are regulated by IRF7, we have performed DNA microarray in 3T3-L1 adipocytes differentiated from precursor cells infected with retrovirus empty or carrying IRF7. Plasmids (Empty- and IRF7-pMSCV) were kindly provided from Dr. Eguchi at the Okayama University Graduate School of Medicine, Okayama, Japan [Cell Metab. 2008;7: 86-94.]. Infected 3T3-L1 preadipocytes were selected by puromycin treatment and differentiated into adipocytes. 7 days after the induction of adipogenesis, total RNA was isolated.

2020-04-01 | GSE147857 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of the control 3T3-L1 (3T3-L1-NC) and LIGHT (tnfsf14) overexpression 3T3-L1 cells (3T3-L1-LIGHT) before and after differentiation into beige adipocytes

Project description:Purpose: The goals of this study are to compare NGS-derived 3T3-L1-NC transcriptome profiling (RNA-seq) to LIGHT overexpression 3T3-L1 cells and find out the DEGs Methods: mRNA profiles of 3T3-L1-NC and 3T3-L1-LIGHT before and after differentiation into beige adipocytes were generated by deep sequencing, in triplicate, using Illumina HiSeq. The sequence reads that passed quality filters were analyzed at the transcript isoform level. Results: Using an optimized data analysis workflow, we mapped about 6.53 Gb data per sample. The average genome mapping rate is 87.27% and the average gene mapping rate is 80.18%. 18,255 genes were identified in which 17,367 of them are known genes and 1,001 of them are novel genes. 13,086 novel transcipts were identified in which 9,304 of them are previously unknown splicing event for known genes, 1,001 of them are novel coding transcripts without any known features, and the remaining 2,781 are long noncoding RNA. Conclusions: Our study represents the first detailed analysis of the impact of LIGHT on gene expression in process of beige adipocytes biogenesis with biologic replicates, generated by RNA-seq technology.

2018-05-22 | GSE114690 | GEO

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