Project description:We aimed to explore the effects of two, high-polyphenol (AMJ) and low-polyphenol (dAMJ), doses of aronia juice (including polyphenol-free placebo, PLB) and their additive effects on whole transcriptome in peripheral blood mononuclear cells (PBMC) of subjects at cardiovascular risk.
Project description:We combined an iTRAQ-based proteome-level analysis with an RNA sequencing-based transcriptome-level analysis to detect the proteins and genes related to fruit peel colour development during two fruit development stages in the ‘Tunisia’ and ‘White’ pomegranate cultivars.
Project description:The simultaneous gene fungal-fruit expression at appressoria, quiescence and necrotrophic stages in C. gloeosporioides-tomato fruit interaction were characterized by Illumina NGS. Fungal appressoria stage on green fruit showed stage-specific transcription and was accompanied by massive fruit transcriptional defense response. The quiescent fungal transcriptome showed activation of chromatin remodeling genes while the fruit response continued a highly integrated and massive up-regulation of defense genes. The necrotrophic stage showed a dramatic shift in up-regulation of C. gloeosporioides pathogenicity factors and a susceptive fruit response that shows activation of the salicylic acid pathway culminating in cell death and anthracnose disease. Simultaneous fungal-fruit transcriptome analysis deepens our perception of the unfolding fungal-fruit arms and defenses race.
Project description:Transcriptome comparsions and integrated systems analysis were used to identify candidate genes associated with fruit acidity control and gene coexpression subnetworks involved in fruit acid accumulation. mRNA profiles of fruit tissues at two stages (45 days postanthesis (DPA); and 142 DPA) from four varieties with differing fruit acidity.
Project description:Transcriptome comparsions and integrated systems analysis were used to identify candidate genes associated with fruit acidity control and gene coexpression subnetworks involved in fruit acid accumulation.
Project description:Purpose: transcriptome sequencing of Conopomorpha sinensis Methods: high-through Illumina HiSeqTM 2000 Results:66017 transcripts,35383 unigenes Conclusions:This study provided valuable transcriptome data for the litchi fruit borer, which was the first fundamental genomic basis for exploiting gene resources from the litchi fruit borer
Project description:[original title] Understanding the complexity of fruit ripening by transcriptome analysis of rin mutant fruit and in silico analysis of promoters of differentially regulated genes A tomato MADS-box transcription factor, LeMADS-RIN, controls fruit ripening and mutation in this gene results in non-ripening phenotype of fruit. This mutation down-regulates certain ripening related ethylene responses, however, other ethylene responses are normal. A complete understanding of this mutation and its effect on fruit transcriptome during ripening is not clear. In this study, microarray analysis has been used to investigate the influence of rin mutation on fruit transcriptome at different stages of ripening. A total of 2,398 genes were found to be differentially expressed in wild type fruit pericarp, which on cluster analysis indicated a major shift in their expression profiles in rin mutant fruit. A total of 1,802 genes were found to be differentially expressed between wild type and rin mutant fruits and 17% of these genes encoded regulatory elements, suggesting that mutation in LeMADS-RIN results in disturbance in the regulatory transcriptional networks during ripening. Since LeMADS-RIN has been reported to bind to the CArG box of LeACS2 promoter, in-silico analysis of 51 putative promoter sequences of the genes, that showed ripening associated up-regulation in wild type but showed impairment in up-regulation in rin mutant fruit during ripening, were searched for presence of CArG box along with ethylene and auxin responsive elements. The study revealed that only 24 putative promoter sequences harbor LeMADS-RIN specific CArG box suggesting an alternative mode of regulation by LeMADS-RIN for CArG box deficient genes. Three chronological stages of tomato (Solanum lycopersicon) fruit ripening were compared between wild type and rin mutant
