Project description:We carried out genome wide transcriptome analyses in different organs and developmental stages of the olive tree using the NimbleGen Array oligonucleotide probe sets. Cluster analyses of the genes showed that samples collected from different organs could be sorted into separate groups. The nutritional control had a particularly remarkable impact on the alternate bearing for O. europaea, as shown by the differential expressions of transcripts under different developmental phases and organs Six sample sets were prepared for array analyses: i) unripe fruit (UF); ii) ripe fruit (RF); iii) “on-year” mature leaf (November sample, ON-M); iv) “on-year” juvenile leaf (July sample, ON-J); v) “off-year” mature leaf (November samples, OFF-M); and vi) “off-year” juvenile leaf (July sample, OFF-J).
Project description:Buds were collected at equivalent branch positions and always at the same time of the day. Samples corresponding to May (MAY), June (JUN), July (JUL), September (SEP), November (NOV), January (JAN), March (MAR) and April (APR) buds were analyzed. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Jose Diaz-Riquelme. The equivalent experiment is VV36 at PLEXdb.]
Project description:Twelve inshore and six offshore colonies were reciprocally transplanted during 1 year (July 2017- July 2018) at Florida Keys (location). After this period samples were collected from the field and brought to the Experimental Reef Laboratory facilities (RSMAS, Miami) to be acclimated to 30C during 7 days in six aquaria. Three aquaria were keep under initial conditions for the duration of the experiment (30C) and three aquaria had the temperature increased everyday during 7 days to a final temperature of 32C. A total of 56 samples were collected for RNAseq after 6 days of the temperature treatment and stored at -80C.
Project description:We aimed to identify miRNA regulated by alternate bearing in O. europaea. For this purpose, six olive (Olea europaea L. )(Ayvalık variety) small RNA libraries were constructed from fruits (ripe and unripe) and leaves ("on-year" and "off-year" mature -leaven in November and juvenile - leaven in July plants) and sequenced by high-throughput Illumina sequencing. Bioinformatics analyses of 93,526,915 reads identified 135 conserved miRNA, belonging to 22 miRNA families in olive tree. In addition, 38 novel miRNA were discovered in the datasets. Expression of olive tree miRNA varied greatly among the six libraries, indicating contribution of diverse miRNA in balancing between reproductive and vegetative phases. The differential expression of miRNA was evaluated on the basis of the developmental phase of the samples.
2012-12-18 | GSE42978 | GEO
Project description:Global surveillance of antimicrobial resistance - batch November 2018
Project description:Melon RNA-Seq analysis was used to identify candidate resistance genes and to understand the early molecular processes deployed during melon versus Fusarium oxysporum f.sp. melonis Snyd. & Hans race 1.2 (FOM1.2) interaction in the resistant doubled haploid line NAD as opposed to the susceptible genotype Charentais-T at 24 and 48 hours post inoculation (hpi).
