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Next Generation Sequencing Facilitates Quantitative Analysis of RBM39 knockdown Transcriptomes in T47D cells

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of RBM39 knockdown Transcriptomes in T47D cells

PROVIDER: PRJNA603522 | ENA |

REPOSITORIES: ENA

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Next Generation Sequencing Facilitates Quantitative Analysis of RBM39 knockdown Transcriptomes in T47D cells

Project description:Methods: Total RNA was prepared from control and RBM39 siRNAs transfected in T47D cells and subsequently used for deep sequencing, in duplicates.

2021-04-13 | GSE144376 | GEO

Genome-wide chromatin occupancy of RBM39 and MLL1 in T47D cells

Project description:We report high-throughput profiling of RBM39 and MLL1 chromatin occupancy in T47D cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of RBM39 and MLL1 in T47D cells. We found that more than 4500 regions were cofound by RBm39 and MLL1.

2021-04-13 | GSE144375 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of human breast cancer cell T47D and T47D-TR Transcriptomes

Project description:To gain new insight into the resistance to hormonal therapy in breast cancer, we generated a tamoxifen-resistant human breast cancer cell line T47D-TR by exposing estrogen-sensitive cell line T47D to increasing concentrations of 4-hydroxy tamoxifen (4-OHT) and identified its resistance with different ways. What we interested is the underlying mechanisms of breast cancer tamoxifen resistance which was not reported yet.Here, we detect the RNA sequence of both the two cell lines hoping to find some evidence.

2020-01-28 | GSE125738 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of human breast cancer cell T47D and T47D-TR Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of human breast cancer cell T47D and T47D-TR Transcriptomes

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Inhibition of a pathologic RBM39/MLL1 epigenomic regulatory complex with a dominant-negative peptides disrupts cancer cell transcription, proliferation and survival

Project description:We report high-throughput profiling of RBM39 and MLL1 chromatin occupancy; H3K4me3 marks in T47D Control, T47D RBM39 KD and PME cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of RBM39, MLL1 and H3K4me3 in T47D and PME cells. We found that more than 4500 regions were cofound by RBM39 and MLL1.

2021-04-13 | GSE169277 | GEO

Genome-wide chromatin occupancy of RBM39 and MLL1 in T47D cells

Project description:Genome-wide chromatin occupancy of RBM39 and MLL1 in T47D cells

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Next Generation Sequencing Facilitates Quantitative Analysis of Solanum lycopersicum bud and flower Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Solanum lycopersicum bud and flower Transcriptomes

2021-02-27 | GSE164382 | GEO

T47D shNT versus T47D shRON

Project description:T47D shNT versus T47D shRON

| PRJNA682172 | ENA