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Differential gene responses three days following infarction in the fetal and adolescent sheep heart

ABSTRACT: Differential gene responses three days following infarction in the fetal and adolescent sheep heart

PROVIDER: PRJNA603845 | ENA |

REPOSITORIES: ENA

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Differential gene responses three days following infarction in the fetal and adolescent sheep heart

Project description:Background: There are critical molecular mechanisms that can be activated to induce myocardial repair and in humans this is most efficient during fetal development. The timing of heart development in relation to birth and the size/electrophysiology of the heart are similar in humans and sheep, providing a model to investigate the repair capacity of the mammalian heart and how this can be applied to adult heart repair. Methods: Myocardial infarction was induced by ligation of the left anterior descending coronary artery in fetal (105d gestation when cardiomyocytes are proliferative) and adolescent sheep (6 months of age when all cardiomyocytes have switched to an adult phenotype). An ovine gene microarray was used to compare gene expression in sham and infarcted (remote, border and infarct areas) cardiac tissue from fetal and adolescent hearts. Results: The gene response to myocardial infarction was less pronounced in fetal compared to adolescent sheep hearts and there were unique gene responses at each age. There were also region-specific changes in gene expression between each age, in the infarct tissue, tissue bordering the infarct, and tissue remote from the infarction. In total, there were 880 genes that responded to MI uniquely in the adolescent samples compared with 170 genes in the fetal response, as well as 742 overlap genes that showed concordant direction of change responses to infarction at both ages. Conclusions: In response to myocardial infarction, there were specific changes in genes within pathways of mitochondrial oxidation, muscle contraction, and haematopoietic cell lineages, suggesting that the control of energy utilisation and immune function are critical for effective heart repair. The more restricted gene response in the fetus may be an important factor in its enhanced capacity for cardiac repair.

2020-01-30 | GSE144509 | GEO

Differential gene responses 3 days following infarction in the fetal and adolescent sheep heart.

Project description: Not available

| S-EPMC7099410 | biostudies-literature

Expression data from mouse heart three days after myocardial infarction

Project description:Identification of the genes whose expresion levels are changed by the operation of myocardial infarction in C57BL/6J mice

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Fetal androgen exposure determines adult male health in Ovis aeries during development

Project description:To elucidate the impact of androgen exposure in fetal life on adolescent sheep, fetuses were exposed to either a vehicle control or testosterone propionate. Sheep were then reared to adolescence, and RNA was extracted from the liver to assess the impact of hormone exposure during fetal development.

2019-12-31 | E-MTAB-8032 | biostudies-arrayexpress

RNA seq for Rat's heart infarction

Project description:This project performed BulkRNA sequencing on the myocardial tissue of rats one month after myocardial infarction and attempted to reveal the transcriptome differences in the myocardial infarction area of rats among different groups.

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Identification of Novel miRNAs Involved in Cardiac Repair Following Infarction in Fetal and Adolescent Sheep Hearts.

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Differential Response to Injury in Fetal and Adolescent Sheep Hearts in the Immediate Post-myocardial Infarction Period.

Project description: Not available

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Expression data from fetal sheep immunocytes

Project description:study investigating the initiation of systemic inflammatory signaling in fetuses exposed to TLR-4 agonist lipopolysaccharides from E.coli microarray was used to determine the inflammatory transcriptome in circulating fetal immunocytes fetal sheep at 92d GA were catheterised prior to intraamniotic exposure fo saline or lipopolysaccharides for 48h prior to euthanasia

2015-08-21 | E-GEOD-72240 | biostudies-arrayexpress

Effects of Maternal Energy Intake on Genomic Imprinting in Fetal Sheep

Project description:Background: Genomic imprinting is an epigenetic phenomenon of differential allelic expression based on parental origin. To date a total of 255 imprinted genes have been identified or predicted among all investigated mammal species. However, only 21 have been described in sheep and 11 are annotated in current ovine genome. Results: Here we aim to use DNA/RNA throughput sequencing to identify monoallelically expressed and imprinted genes in organs of day 135 fetal sheep, and 2) to determine whether different levels of maternal energy intake (100% of NRC energy requirement or control, 140% or over-, and 60% or under-fed) influence genomic imprinting. We report strategies to solve technical challenges in the next-generation sequencing data analysis pipeline, including alignment bias of RNA sequencing reads and filtering potential false positives. We identified 80 monoallelically expressed and 18 putatively imprinted genes using the list of 255 stated above as a guide. Five (45.6% of 11) were already known imprinted genes in sheep, the other 13 were known imprinted in other mammals. Sanger sequencing confirmed four putative sheep imprinted genes INPP5F, PLAGL1, CASD1 and PPP1R9A. Among the 13 putative imprinted genes, five localized in the known sheep imprinting clusters of MEST domain on chromosome 4, DLK1/GTL2 domain on chromosome 18 and IGF2/H19 domain on chromosome 21, three were in a novel sheep imprinted cluster on chromosome 4 known in other species as PEG10/SGCE. Additionally, we found that the imprinted genes PHLDA2, SLC22A18, DIRAS3, and IGF2 were differentially expressed, albeit without allelic expression reversal, among the three maternal nutritional groups. Conclusions: Together, our results expanded the sheep imprinted gene list to 34 and demonstrated the influence of maternal diet on fetal imprinting under the conditions studied.

2018-08-29 | GSE111306 | GEO

Thrombopoietin (TPO) protects against heart damage induced infarction in rat model

Project description:In order to examine the mechanism of TPO on cardiac protection against myocardial infarction damage (MI), we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to delineate the TPO cardioprotective mechanism against infarction. MI and TPO induced gene expressions in rat heart were measured at week 4. Two biological replicates were performed for each treatment group.

2012-12-01 | E-GEOD-22489 | biostudies-arrayexpress

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