Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MiR-21 plays a dual role in tumor formation and cytotoxic response in breast tumors

ABSTRACT: miR-21 plays a dual role in tumor formation and cytotoxic response in breast tumors

PROVIDER: PRJNA604816 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

miR-21 plays a dual role in tumor formation and cytotoxic response in breast tumors

Project description:Breast cancer (BrCa) relies on specific microRNAs to drive disease progression. Oncogenic miR-21 is upregulated in many cancers including BrCa and is associated with poor survival and treatment resistance. We sought to determine the role of miR-21 in BrCa tumor initiation, progression and treatment response. In a triple-negative BrCA model, radiation exposure increased miR-21 in both primary tumor and metastases. In vitro, miR-21 knockdown decreased survival in all BrCa subtypes in presence of radiation. The role of miR-21 to BrCa initiation was evaluated by implanting wild-type miR-21 BrCa cells into genetically-engineered mouse models where miR-21 was intact, heterozygous or globally ablated. Tumors were unable to grow in mammary fat pads of miR-21-/- mice, grew in ~50% of miR-21+/- and 100% in miR-21+/+ mice. The contribution of miR-21 in progression and metastases was tested by crossing miR-21-/- mice with mice that spontaneously develop BrCa. Global ablation of miR-21 significantly decreased tumorigenesis and metastases of BrCa while sensitizing tumors to radio- and chemotherapeutic agents by a Fas/FasL-dependent apoptosis. Therefore, targeting miR-21 alone or in combination with various radio or cytotoxic therapies may represent novel and efficacious therapeutic modalities for the future treatment of BrCa patients. We used microarray to identify gene expression associated with miR-21 function in breast development and metastasis.

2021-03-10 | GSE144773 | GEO

miR-21 Plays a Dual Role in Tumor Formation and Cytotoxic Response in Breast Tumors.

Project description: Not available

| S-EPMC7924198 | biostudies-literature

SMAD4 plays acritical role in CD8 T cell cytotoxic function

Project description:SMAD4 plays acritical role in CD8 T cell cytotoxic function

| PRJNA792409 | ENA

SMAD4 plays acritical role in CD8 T cell cytotoxic function [ChIP-seq]

Project description:SMAD4 plays acritical role in CD8 T cell cytotoxic function [ChIP-seq]

| PRJNA792408 | ENA

SMAD4 plays acritical role in CD8 T cell cytotoxic function [RNA-seq]

Project description:SMAD4 plays acritical role in CD8 T cell cytotoxic function [RNA-seq]

| PRJNA792411 | ENA

miR-21 is critical in stress response and facilitates breast cancer development and metastasis [miRNA array]

Project description:miR-21 is upregulated in advanced tumor stages, distal metastasis, and poor survival and in response to cytotoxic stress, but is also known to confer resistance to radiation and chemotherapy. We discovered that global ablation of miR-21 significantly decreased tumorigenesis and metastatic spread of breast cancer, while sensitizing breast carcinomas to cytotoxic agents by evoking Fas/FasL-dependent apoptosis. Targeting miR-21 may represent a novel and efficacious therapeutic modality either alone or in combination with standard oncologic treatments.

2022-02-01 | GSE145344 | GEO

MiR-21 function in T-lymphocytes

Project description:T-lymphocyte activation is efficiently mimicked in vitro by treatment with anti CD3 / anti CD28 antibodies. We report miR-21 induction upon CD3/CD28 stimulation of primary T-lymphocytes. In order to assess the function of miR-21 in T-lymphocytes we interfered with miR-21 function by lentiviral transduction of a miR-21 sponge construct. MRNA profile of miR-21 sponge and control transduced T-lymphocytes 48hrs after stimulation.

2014-10-15 | GSE37213 | GEO

SMAD4 plays acritical role in CD8 T cell cytotoxic function [RNA-seq]

Project description:Transforming growth factor-beta (TGF-β) restrains cytotoxic immune response to maintain self-tolerance and to promote tumor immune evasion. Yet how SMAD4, a central transcription factor component of TGF-β signaling, regulates CD8+ T cell function remains unclear. Here we have demonstrated SMAD4 played a critical role in promoting CD8+ T cell activation and cytotoxic function. SMAD4-mediated transcriptional regulation of CD8+ T cell activation and cytotoxicity is regulated by T-cell receptor (TCR) signaling pathway rather than TGF-β signaling pathway. We described a new mechanism that in TCR-mediated intracellular signal propagation, SMAD4 markedly translocated into the nucleus, upregulated genes that encoding TCR complex subunits and cytotoxic molecules in CD8+ T cells, reinforced the TCR-activation signals through a positive feedback loop. And in this signaling, SMAD4 is phosphorylated by ERK at Ser367 residue. Our study thus demonstrates an essential role of SMAD4 in promoting CD8+ T cell mediated cytotoxic immune responses.

2022-07-12 | GSE192621 | GEO

SMAD4 plays acritical role in CD8 T cell cytotoxic function [ChIP-seq]

2022-07-12 | GSE192620 | GEO

miR-21 OVA Lung

Project description:Lungs from miR-21+/+ and miR-21-/- mice sensitized with OVA/Alum and challenged with either saline or OVA.

2011-08-25 | E-MEXP-3119 | biostudies-arrayexpress