Project description:We performed high-throughput RNA sequencing to characterize possible differences in the transcriptome of murine liver from both 12 months old EndoV and wildtype mice mice fed a regular diet.
Project description:RNA from wt and SIN1 knock-out MEF cell lines were compared Using affymetrix murine gene chip, the effects of SIN1 ablation on global gene transcription were assessed
Project description:Goal: elucidate transcriptomic changes upon knock-out of components of the FERRY complex Methods: RNA extraction from HeLa wildtype and fy-1, fy-2, fy-4 and fy-5 knock-out celllines and subsequent RNASeq Results: We observed differences in the transcriptome of all four knock-out cell lines Conclusions: In the Analysis we focused on genes that were differentially expressed in all four KO cell lines or upon KO of fy-1 and fy-2.
Project description:To investigate the functional importance of a nucleoside transporter, the mENT1 (Slc29a1) was knocked out in mice. The gene expression profile was compared between wildtype and mENT1 knock out mice in two tissues.
Project description:RNA from wt and SIN1 knock-out MEF cell lines were compared Using affymetrix murine gene chip, the effects of SIN1 ablation on global gene transcription were assessed RNA from wild type and SIN1 knock out cells was purified and differences in gene expression between the two sets. MEFs were derived from C57BL/6 (Jacinto et. al. Cell. 2006 Oct 6;127(1):125-37. Epub 2006 Sep 7
Project description:(1) While it is known that redox-regulation in chloroplasts is vital for functional photosynthesis, the interplay of different redox-cascades in balancing reactive oxygen species (ROS) with metabolic regulation via thiol switching is still partly unresolved. We investigate the importance of the glutathione reductase isoform maintaining a highly reducing stromal glutathione redox potential (EGSH). (2) Using the model moss Physcomitrella patens, we knocked-out the plastid/mitochondrial GR isoform. We generated a sensor line expressing plastid-targeted redox-sensitive GFP to monitor stromal EGSH in vivo, and compared protein abundances between wildtype (WT) and Δgr1 knock-out mutants by a metabolic labelling approach. (3) On the organelle level, we find that the absence of PpGR1 leads to a shift of stromal EGSH to less reducing values that is not rescued in the light, whereas WT EGSH was clearly responsive to light. On the plant level, growth and photosynthetic performance were decreased with increasing light intensities while ascorbate and zeaxanthin levels were elevated. Proteomics showed an induction of proteins involved in plastid protein repair and degradation. (4) Our results indicate a limited cross-talk between plastid thioredoxin and glutathione redox systems and show that glutathione reductase is necessary for efficient photosynthesis under stress and non-stress conditions.
Project description:To further characterize differential expression of miRNA and mRNA levels in livers of 12 weeks old male Tax1BP1 wildtype and knock-out mice, the untreated mice were sacrificed and miRNA and mRNA levels were determined