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FGFR3 is a positive regulator of osteoblast expansion and differentiation during zebrafish skull vault development

ABSTRACT: FGFR3 is a positive regulator of osteoblast expansion and differentiation during zebrafish skull vault development

PROVIDER: PRJNA605419 | ENA |

REPOSITORIES: ENA

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FGFR3 is a positive regulator of osteoblast expansion and differentiation during zebrafish skull vault development

Project description:In order to study FGFR3’s role in cranial vault development, we generated the first fgfr3 loss-of-function zebrafish (fgfr3lof1/lof1). The mutant fish exhibited major changes in the craniofacial skeleton, with a lack of sutures, abnormal frontal and parietal bones, and the presence of ectopic bones. In order to define cellular and molecular processes responsible for this phenotype, we performed single-cell RNA sequencing of cranial vault cells from fgfr3lof1/lof1 and fgfr3+/+ zebrafish. Our data allow us to define transcriptomic profile of each osteogenic subpopulation involved in cranial vault formation and we revealed a defect in osteoblast differentiation associated with changes in the extracellular matrix. These findings demonstrate that fgfr3 is a positive regulator of osteogenesis and we conclude that changes in the extracellular matrix within growing bone impair cell-cell communication, mineralization, and new osteoblast recruitment.

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Increased FGF8 Signaling Promotes Chondrogenic Rather Than Osteogenic Development in the Embryonic Skull

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