ABSTRACT: Single-cell, paired heavy- and light-chain immunoglobulin sequencing of B cells from peripheral blood and synovial tissue of rheumatoid arthritis subjects
Project description:Paired synovial and peripheral blood CD14+ cells were obtained from patients with active rheumatoid arthritis and compared to each other
Project description:Total RNA sequencing was performed on samples isolated from joint synovial biopsies from subjects with and without rheumatoid arthritis
Project description:Total RNA sequencing was performed on samples isolated from joint synovial biopsies from subjects with rheumatoid arthritis before and after 6 months of triple DMARD treatment
Project description:Lyme disease (Borrelia burgdorferi infection) is increasingly recognized as a significant source of morbidity world-wide. Here, we investigated B cell responses to Lyme disease through molecular identifier-enabled antibody heavy chain sequencing of bulk B cells from PBMCs. Single-cell immunoglobulin sequencing of paired heavy- and light-chain genes from this project will also be separately deposited. Additional information regarding patient characteristics and overlap with other data from the SLICE study is available upon request.
Project description:CD14+ monocytes sorted from the synovial fluid or peripheral blood of rheumatoid arthritis patients were analyzed by full transcriptome microarray analysis. Monocytes from healthy control samples (peripheral blood) were also profiled.
Project description:We report changes in gene expression that take place in human neutrophils following migration from peripheral blood to synovial joints in patients with rheumatoid arthritis
2020-11-24 | GSE154474 | GEO
Project description:Paired Heavy and Light Chain Immunoglobulin Reconstruction in Single-Cell RNA-Seq Data
