Project description:Host-microbe associations underlie many key processes of host development, immunity, and life history. Yet, none of the current research on the central model species Caenorhabditis elegans considers the worm’s natural microbiome. Instead, almost all laboratories exclusively use the canonical strain N2 and derived mutants, maintained through routine bleach sterilization in monoxenic cultures with an E. coli strain as food. Here, we characterize for the first time the native microbiome of C. elegans and assess its influence on nematode life history characteristics via transcriptomics.
Project description:To determine gene expression differences in the olfactory epithelium of sea lamprey between sequential yet behaviorally distinct adult life history stages
Project description:In this study transcriptomic data of three life history stages of Orciraptor agilis was generated: 1) Gliding cells in absence of food ('gliding'), 2) Cells attached to the cell wall of its algal prey during perforation ('fattacking'), 3) Cells after acquisition of the algal plastid material ('digesting'). Furthermore, RNA-seq of the algal prey Mougeotia sp. was also performed. A de novo transcriptome assembly of the algal reads was performed in order to identify and substract algal reads of the Orciraptor samples by mapping the Orciraptor reads to the algal transcriptome. After this filtering step the remaining Orciraptor reads from all libraries were pooled for a de novo transcriptome assembly of Orciraptor agilis. This transcriptome was the basis for a comparative transcriptomic study in which transcript expression was compared between the three life history stages.
Project description:Comprehensive RNA sequencing was performed on a laboratory colony of B. dorsalis with a focus on attempting to capture as many genes in the sequencing from throughout the entire developmental life history. De novo assembly and analysis of the resulting sequence One sample each for the egg, larvae, pupae, adult male, adult female and mated female life stages was sequenced.
Project description:Here we present LC-MS/MS proteomic datasets of Heliocidaris erythrogramma, Heliocidaris tuberculata, and Lytechinus variegatus eggs and larvae. We find dramatic proteomic differences likely associated with life history evolution and between developmental stages. This study provides a complimentary dataset to previous transcriptomic analyses of the same three sea urchin species.
Project description:To determine gene expression differences in the olfactory epithelium of sea lamprey between sequential yet behaviorally distinct adult life history stages 2 samples: parasitic adults removed from fish in northern Lake Huron and Lake Michigan in February and March, and reproductive adults collected from Lake Huron and Lake Michigan tributaries in June
Project description:In this study our research group has completed the metabolome sequencing of Thelephora ganbajun Zang in three key developmental stages, including mycelium, primordium and fruiting body, aiming to explore the specific substances or special mechanisms that may affect the development of ectomycorrhiza of strain 46 in its entire life history indifferent developmental stages, and to conduct dynamic real-time monitoring of the gene expression products
2023-12-18 | PXD047922 | iProX
Project description:Microbiome of different life stages of the anhydrobiotic midge
