Project description:In this study we want to compare the gene expression profile of CD44+CXCR6+ CD8+ T cells from the liver of ND and CD-HFD mice with hepatic CD44+CXCR6neg and splenic CD44+ CX3CR1neg CD8 T cells of ND and CD-HFD mice. This comparison will reveal transcriptional signatures of hepatic CD44+CXCR6+ CD8+ T cells in CD-HFD responsible for causing liver pathology in NASH.
Project description:We used microarray analysis to identify specific molecular mechanisms controlling Th17 cell differentiation in HFD mice Memory phenotype CD4 T cells derived from ND or HFD mice were selected for RNA extraction and hybridization on Affymetrix microarrays.
Project description:Transcriptional profiling for screening olfactory receptor expressions in C57BL/6J mouse liver and adipose tissue under either ND and HFD
Project description:To investigate the effects of a high-fat diet (HFD) on the skin, RNA-seq was performed on the ear skin after 8 weeks of normal diet (ND) and HFD-fed mice. We also examined the effects of a HFD on irritant dermatitis by PMA.
Project description:High-throughput scRNA-seq transcriptionally profiled an estimated 15093 cells from CD islets and 18906 from HFD islets. The mean number of reads per sequenced cell was 43,301 for CD islets and 33,867 for HFD islets, while the median number of genes per sequenced cell was 2105 and 1775, respectively.
Project description:Genomic DNA was extracted from tumors that developed in C57BL/6 mice fed with CD-HFD for 12 months alone or fed with CD-HFD for 12 months followed by 8 weeks of treatment with anti-PD-1. DNA extracted from five pooled tail-clips of C57BL/6 mice was used as reference DNA.
Project description:We perform scRNA-seq in the livers of C57BL/6 mice fed a CD-HFD or control diet for 3 months (NAFLD), 6 months (NASH) or 15 months (HCC)
Project description:Pyrosequencing analysis of microbial communities of mice fed normal diet
(ND), high fat diet (HFD) or HFD supplemented with KFLAB (HFD-KFLAB) using 454 GS
FLX Titanium System
Project description:After feeding CD-HFD diet for 15 month, tumors from WT and FABP5 hepatocyte KO mice were collected and digested with collagenase to generate single cell suspension before proceeding with 10X Single Cell RNA-Sequencing.