Project description:Intravasation, vascular dissemination and metastasis of malignant tumor cells require their passage over the vascular wall which is commonly composed of pericytes (mural cells) and the endothelial cell barrier. We currently decided to investigate the relative contribution of these cell types for B16F10 melanoma metastasis in mice using an experimental model of Shb gene inactivation. RNAseq of tumor endothelial cells from these mice revealed changes in cellular components such as adherens junctions and focal adhesions by gene ontology analysis that were in line with the observed effects on leakage and junction morphology.
Project description:BMP2 treatment significantly reduces growth of B16F10 melanoma spheroids. This experiment analyses the transcriptome changes in B16F10 cells treated with or without BMP2.
Project description:We evaluated the changes in the transcriptome of spleen and bone marrow isolated myeloid cells in healthy and melanoma bearing animals and melanoma cell lines, B16F10 and YUMM3.3 using RNA-sequencing technologies.
Project description:Major transcriptional changes (300 differentially expressed genes) take place upon downregulation of Lamin A/C in B16F10 melanoma cells
Project description:We evaluated the changes in the transcriptome of a melanoma cell line, B16F10, and spleen isolated murine T cells when in the presence of hypochlorous acid (HOCl) using RNA-sequencing technologies.
Project description:Global gene expression profiling reveals a potential anti-melanoma effect of SQ-diEG in B16F10 cells. Squalene (SQ) was considered as a promising natural agent in anti-cancer treatment due to its strong antioxidant and anti-inflammatory activity; however, its pharmacological value has been largely underestimated because of its poor solubility and bioavailability. To adress this problem, a novel amphiphilic SQ derivative which bearing ethylene glycol oligomers was synthesized and was used as a permeation enhancer in this study to check its potential effect on anti-melanoma using B16F10 cells B16F10 were murine melanoma cells line, treated with 2.5 µM and 40 µM with all samples for 48 h. Microarray gene expression profiling was conducted for two biological replicates
Project description:Gene expression profiling reveals a potential role of Butyroside D in melanogenesis inhibition in B16F10 cells. B16F10 cells were murine melanoma cell line, treated with 2 μM Butyroside D for 48 h. Microarray gene expression profiling was conducted for two biological replicates
Project description:Gene expression profiling reveals a potential role of Butyroside D in melanogenesis inhibition in B16F10 cells. B16F10 cells were murine melanoma cell line, treated with 0.2 μM Butyroside D for 48 h. Microarray gene expression profiling was conducted for two biological replicates
Project description:Under the hypothesis that subset of parental cells may already harbor metastasis potential, which leads progression of metastasis, we investigated single-cell expression of parental B16 melanoma cell (B16F0) and its highly metastatic subclone (B16F10) (N=4,156) using single-cell RNA sequencing