Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MicroRNA profiling of exosomes derived from human pulmonary artery smooth muscle cells under PDGF stimulation

ABSTRACT: microRNA profiling of exosomes derived from human pulmonary artery smooth muscle cells under PDGF stimulation

PROVIDER: PRJNA607447 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

microRNA profiling of exosomes derived from human pulmonary artery smooth muscle cells under PDGF stimulation

Project description:Analysis of exosomal microRNAs secreted by PDGF-stimulated human pulmonary artery smooth muscle cells. Results increase our understanding of exosome-mediated crosstalk between vascular cells under a pathological condition.

2020-04-22 | GSE145508 | GEO

Transcriptional profiling of human pulmonary artery smooth muscle cells under hypoxia

Project description:Analysis of hypoxia-exposed human pulmonary artery smooth muscle cells to identify the commonly regulated genes by hypoxia. Results provide insight into the regulatory mechanism of hypoxic responses in vascular smooth muscle cells.

2019-09-25 | GSE118362 | GEO

microRNA profiling of human pulmonary artery smooth muscle cells under hypoxia

Project description:Analysis of hypoxia-exposed human pulmonary artery smooth muscle cells to identify the commonly regulated microRNAs by hypoxia. Results provide insight into the regulatory mechanism of hypoxic responses in vascular smooth muscle cells.

2019-09-25 | GSE118361 | GEO

2 lines of pulmonary artery smooth muscle cells metabolic profile (122 vs 3633C central metabolic profile)

Project description:2 lines of pulmonary artery smooth muscle cells which has been snap frozen during active growth phase

2015-06-06 | ST000180 | MetabolomicsWorkbench

Human pulmonary artery smooth muscle cells under hypoxia

Project description:Human pulmonary artery smooth muscle cells under hypoxia

| PRJNA485330 | ENA

SILAC analysis of PDGF-treated primary bladder smooth muscle cells

Project description:SILAC analysis of human primary bladder smooth muscle cells treated with platelet-derived growth factor for 0, 4, and 24 h. Platelet-derived growth factor-BB (PDGF-BB) is a mitogen and motogen that has been implicated in the proliferation, migration and synthetic activities of smooth muscle cells (SMC) that characterize pathologic tissue remodeling in hollow organs. To explore the signals induced by PDGF on a global scale, we performed expression profiling and quantitative proteomics analysis of PDGF-treated human visceral SMC. 1695 genes and 241 proteins were identified as differentially expressed in PDGF-treated primary bladder SMC versus non-treated cells. Analysis of gene expression data revealed MYC, JUN, EGR1, MYB and RUNX1 as the transcription factors most significantly networked with upregulated genes; DDIT3, NFAT5, and SOX5 were most networked with downregulated genes. For protein identification and quantification, raw mass spectrometric data were analyzed with MaxQuant software (version 1.0.13.13). The parameters were set as follows. In the Quant module, SILAC triplets was selected; oxidation (M) and acetyl (Protein N-term) were set as variable modification; carbamidomethyl (C) was set as fixed modification; concatenated IPI human database (version 3.52) (74,190 forward sequences and 74,190 reverse sequences) was used for database searching; all other parameters were default. Tandem mass spectra were searched by Mascot (version 2.2.0.4) (Matrix Science, Boston, MA). In the Identify module, all parameters were default, except that maximal peptide posterior error probability was set as 0.05. False discovery rates for protein and peptide identifications were both set at 0.01.

2014-08-04 | PXD000624 | Pride

Microarray to identify miR-143 targets in pulmonary artery smooth muscle cells.

Project description:Pulmonary artery smooth muscle cells were either mock transfected, transfected with scramble control or transfected with pre-miR-143. After 24 hours the cells were harvested with Qiazol and processed for a microarray experiment. The experiment was performed in order to identify potential targets of miR-143.

2015-06-26 | E-MTAB-3567 | biostudies-arrayexpress

Profiling of microRNAs associated with Nucleolin (NCL) in human pulmonary artery smooth muscle cells

Project description:Analysis of miRNAs associated with NCL via RNA immunoprecipitation in pulmonary artery smooth muscle cells using NCL antibodies to identify a distinct set of miRNAs regulated by NCL. Results provide insight into the regulatory mechanism of RNA binding proteins in vascular smooth muscle cells.

2023-03-20 | GSE184972 | GEO

RBPMS knockdown and overexpression in rat PAC1 pulmonary artery smooth muscle cells (SMCs)

Project description:Investigation of RBPMS role in post-transcriptional control of mRNAs in rat PAC1 pulmonary artery smooth muscle cells (SMCs). PolyA mRNA-Seq was carried out after RBPMS knockdown in differentiated PAC1 cells and after inducible RBPMS-A overexpression in dedifferentiated (proliferative) PAC1 cells.

2019-06-18 | GSE127799 | GEO

Gene expression profiling regulated by PDGF in pBSMCs

Project description:To explore global molecular changes in smooth muscle in response to PDGFR activation, primary human bladder smooth muscle cells were treated with 1 nM PDGF-BB (hereafter PDGF) for 0, 4 or 24 h. Total RNA were prepared, and analyzed using expression profiling, and subjected to bioinformatic and functional interrogation. To identify molecular signatures of bladder smooth muscle peturbed by PDGF, primary human bladder smooth muscle cells were treated with 1 nM PDGF-BB (hereafter PDGF) for 0, 4 or 24 h.

2014-10-06 | E-GEOD-52488 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data