Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Rodent malaria parasite RNA does not affect mouse BeadChip results

ABSTRACT: Rodent malaria parasite RNA does not affect mouse BeadChip results

PROVIDER: PRJNA607775 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Rodent malaria parasite RNA does not affect mouse BeadChip results

Project description:Rodent malaria parasite RNA hybridized on Illumina Mouse WG-6 v2.0 Expression BeadChip To investigate whether parasite RNA interfere with mouse beadchip analaysis. Malaria parasite resides in red blood cell, therefore RNA isolated from whole infected blood contains host RNA as well as parasite RNA

2020-04-30 | GSE145634 | GEO

Simultaneous host parasite RNA sequencing of rodent malaria infection time course

Project description:We have shown previously (Reid & Berriman, NAR, 2012) that by simultaneously examining host and parasite gene expression over the course of infection we can determine pairs of genes involved in host-parasite interaction. Here we are producing a high quality dataset which will specifically allow us to exploit this finding to identify genes involved in malaria host-parasite interaction. This has been done in collaboration with Jean Langhorne at NIMR. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2013-05-13 | E-ERAD-136 | biostudies-arrayexpress

Transcriptome Survey of Malaria Parasite Liver Stages

Project description:To facilitate pre-eythrocytic malaria vaccine and drug target identification, a comprehensive transcriptome analysis of the parasites liver stages (LS) was undertaken. Green fluorescent protein-tagged Plasmodium yoelii (PyGFP) was used to isolate LS-infected hepatocytes from the rodent host. Genome-wide LS gene expression was profiled and compared to other parasite life cycle stages. The analysis reveals ~2000 genes active during LS development. Keywords: Stage comparison, time course

2008-01-07 | GSE8125 | GEO

Expression profiling of hemocytes from Anopheles gambiae after malaria parasite infection

2009-10-30 | GSE17866 | GEO

Expression profiling of hemocytes from Anopheles gambiae after malaria parasite infection

Project description:Insect hemocytes mediate important cellular immune responses including phagocytosis and encapsulation, and also secrete immune factors such as opsonins, melanization factors, and antimicrobial peptides. In Anopheles, they contribute to the defense against malaria parasite invasion during the early sporogonic cycle. We used microarrays to identify if and to what degree circulating hemocytes have altered global expression profiles after infection with the rodent malaria parasite, Plasmodium berghei Hemocytes were isolated 24-28h after infection using the infectious EGFP-CON P. berghei strain (experiment) or an invasion-deficient, Circumsporozoite- and TRAP-related protein (CTRP) knockout strain with the same genetic background as GFP-CON (CTRPko/GFP, control).

2009-10-29 | E-GEOD-17866 | biostudies-arrayexpress

Proteome of the rodent malaria parasite Plasmodium berghei liver stage merosomes

Project description:We use the Plasmodium berghei rodent model to characterize the proteome of the final phase of liver stage development, the merosomes, packets of hepatic merozoites that bud from the host hepatocyte to initiate the blood stage of malaria. Plasmodium berghei WT ANKA strain was used to infect HepG2 hepatoma cells. Samples were fractionated by strong cation exchange, and nano-LC Orbitrap mass spectrometry was used to perform untargeted proteomic profiling of 3 biological replicates. Data was processed using MaxQuant and LFQ. Additional searches were performed to identify peptides from cleaved acetylated PEXELs (protein export elements) to identify proteins putatively exported to the host hepatocyte during liver stage development.

2019-03-25 | PXD010559 | Pride

Simultaneous host parasite RNA sequencing of mosquito transmistted rodent malaria in two host backgrounds

Project description:The aim of this study is to explore the role of differences in host genetic background which will supplement our current analysis of a timecourse of P.chabaudi infection in C57BL/6 mice over the peak of parasitemia. We have also published a paper showing that infections using mosquito-transmitted parasites have a quite different effect on the host immune system (Spence et al, Nature, 2013) and using this study we can compare the effects in host and parasite of serially blood passaged parasites versus mosquito transmitted parasites. This is in collaboration with Jean Langhorne at NIMR.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2014-03-04 | E-ERAD-221 | biostudies-arrayexpress

Impact of dietary restriction (DR) on the transcriptome of the rodent malaria parasite Plasmodium berghei [microarray]

Project description:The aim was to understand the molecular basis of the parasite response to DR. Transcriptomic analysis of synchronized wildtype parasites, collected with 4h intervals, from mice under the two dietary conditions. We found that WT parasites on DR and control diets have different transcriptomes, with a remarkable general repression of transcription in DR.

2017-07-14 | GSE69628 | GEO

The Variantome of the malaria parasite Plasmodium falciparum.

Project description:We compared transcript levels at several points along the asexual blood cycle between 21 P. falciparum lines. These 21 parasite lines are organized in 4 sets of parasite lines, with all parasite lines within a set sharing a clonal origin. We compared transcript levels within each set. We also performed comparative genome hybridization (CGH) for all 21 parasite lines.

2012-02-07 | E-MTAB-673 | biostudies-arrayexpress

Rodent malaria genome project

Project description:Sequence data and draft assemblies and annotation for a set of rodent malaria species.

| PRJEB12003 | ENA