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Bcl11b binding and transcriptome analysis of Bcl11b-deficient MAIT cells at steady state and infection

ABSTRACT: Bcl11b binding and transcriptome analysis of Bcl11b-deficient MAIT cells at steady state and infection

PROVIDER: PRJNA608197 | ENA |

REPOSITORIES: ENA

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Transcriptome analysis of Bcl11b-deficient MAIT cells at steady state and infection

Project description:Transcriptome analysis of Bcl11b-deficient MAIT cells at steady state and infection

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Transcriptome analysis of Bcl11b-deficient MAIT cells at steady state and infection

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Project description:T regulatory (Treg) cells have been studied in depth since their discovery for their potential use in therapies of autoimmune diseases. Treg cells have a suppression program that includes surface molecules CD25 (IL2R), cytotoxic T-lymphocyte associated protein 4 (CTLA4), and glucocorticoid-induced TNFR family (GITR) to limit aberrant and excessive inflammatory immune responses. We have shown that Bcl11b can bind to the CNS2 region in Foxp3 as well as the gene loci of those essential surface molecules for Treg suppression. Furthermore, we have identified a subset of Foxp3-independent genes in Treg cells directly regulated by Bcl11b binding. Bcl11b also directly represses expression of innate molecules such as transcription factors PU.1 and ID2 in Treg cells. Finally, we have also shown that removal of Bcl11b accelerates apoptosis in Treg cells as cleaved caspase 3 levels were significantly elevated in Bcl11b KO Treg cells when compared with WT Treg cells.

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Project description:Naïve CD44low CD25low CD8+ T cells from Bcl11bF/F/dLck-iCre and wild type mice at steady state were sorted at 90% purity, RNA was extracted and profile for mRNA expression to identify mRNAs differentially expressed in Bcl11b-/- naïve CD8+ T cells versus wild type. Naïve CD44low CD25low CD8+ T cells purified from Bcl11bF/F/dLck-iCre and wild type mice and investigated for mRNA expression

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Bcl11b binding and transcriptome analysis of Bcl11b-deficient MAIT cells at steady state and infection

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-03-22 | GSE145779 | GEO