Project description:Alternative splicing (AS) is a widely observed phenomenon in eukaryotes that plays a critical role in development and stress responses. In plants, the large number of RNA-seq datasets in response to different environmental stressors can provide clues for identification of condition-specific and/or common AS variants for preferred agronomic traits. We report RNA-seq datasets (350.7 Gb) from Capsicum annuum inoculated with one of three bacteria, one virus, or one oomycete and obtained additional existing transcriptome datasets. In this study, we investigated the landscape of AS in response to environmental stressors, signaling molecules, and tissues from 425 total samples comprising 841.49 Gb. In addition, we identified genes that undergo AS under specific and shared stress conditions to obtain potential genes that may be involved in enhancing tolerance to stressors. We uncovered 1,642,007 AS events and identified 4,354 differential alternative splicing (DAS) genes related to environmental stressors, tissues, and signaling molecules. This information and approach provide useful data for basic-research focused on enhancing tolerance to environmental stressors in hot pepper or establishing breeding programs.
Project description:Alternative splicing (AS) is a widely observed phenomenon in eukaryotes that plays a critical role in development and stress responses. In plants, the large number of RNA sequencing (RNA-seq) datasets in response to different environmental stressors can provide clues for identification of condition-specific and/or common AS variants for preferred agronomic traits. In this study, we report five RNA-seq datasets (350.7 Gb) inoculated with one of three bacteria, one virus, or one oomycete. In addition, we obtained existing transcriptome datasets in response to abiotic and biotic stressors, phytohormones, and various developmental stages. From 428 total samples comprising 841.49 Gb, we identified the AS landscape in C. annuum. We further uncovered 1,704,387 AS events through differential AS analysis and identified 6,280 differential alternative splicing (DAS) genes related to environmental stressors, developmental stages, and phytohormones. This information and approach will enable comparative analysis of differential AS from RNA-seq datasets. Additionally, our findings provide useful data for basic-research programs focused on enhancing tolerance to environmental stressors in hot pepper or establishing breeding programs.
Project description:Alternative splicing (AS) is a widely observed phenomenon in eukaryotes that plays a critical role in development and stress responses. In plants, the large number of RNA sequencing (RNA-seq) datasets in response to different environmental stressors can provide clues for identification of condition-specific and/or common AS variants for preferred agronomic traits. In this study, we report five RNA-seq datasets (350.7 Gb) inoculated with one of three bacteria, one virus, or one oomycete. In addition, we obtained existing transcriptome datasets in response to abiotic and biotic stressors, phytohormones, and various developmental stages. From 428 total samples comprising 841.49 Gb, we identified the AS landscape in C. annuum. We further uncovered 1,704,387 AS events through differential AS analysis and identified 6,280 differential alternative splicing (DAS) genes related to environmental stressors, developmental stages, and phytohormones. This information and approach will enable comparative analysis of differential AS from RNA-seq datasets. Additionally, our findings provide useful data for basic-research programs focused on enhancing tolerance to environmental stressors in hot pepper or establishing breeding programs.
Project description:Alternative splicing (AS) is a widely observed phenomenon in eukaryotes that plays a critical role in development and stress responses. In plants, the large number of RNA sequencing (RNA-seq) datasets in response to different environmental stressors can provide clues for identification of condition-specific and/or common AS variants for preferred agronomic traits. In this study, we report five RNA-seq datasets (350.7 Gb) inoculated with one of three bacteria, one virus, or one oomycete. In addition, we obtained existing transcriptome datasets in response to abiotic and biotic stressors, phytohormones, and various developmental stages. From 428 total samples comprising 841.49 Gb, we identified the AS landscape in C. annuum. We further uncovered 1,704,387 AS events through differential AS analysis and identified 6,280 differential alternative splicing (DAS) genes related to environmental stressors, developmental stages, and phytohormones. This information and approach will enable comparative analysis of differential AS from RNA-seq datasets. Additionally, our findings provide useful data for basic-research programs focused on enhancing tolerance to environmental stressors in hot pepper or establishing breeding programs.
Project description:Alternative splicing (AS) is a widely observed phenomenon in eukaryotes that plays a critical role in development and stress responses. In plants, the large number of RNA sequencing (RNA-seq) datasets in response to different environmental stressors can provide clues for identification of condition-specific and/or common AS variants for preferred agronomic traits. In this study, we report five RNA-seq datasets (350.7 Gb) inoculated with one of three bacteria, one virus, or one oomycete. In addition, we obtained existing transcriptome datasets in response to abiotic and biotic stressors, phytohormones, and various developmental stages. From 428 total samples comprising 841.49 Gb, we identified the AS landscape in C. annuum. We further uncovered 1,704,387 AS events through differential AS analysis and identified 6,280 differential alternative splicing (DAS) genes related to environmental stressors, developmental stages, and phytohormones. This information and approach will enable comparative analysis of differential AS from RNA-seq datasets. Additionally, our findings provide useful data for basic-research programs focused on enhancing tolerance to environmental stressors in hot pepper or establishing breeding programs.
Project description:Alternative splicing (AS) is a widely observed phenomenon in eukaryotes that plays a critical role in development and stress responses. In plants, the large number of RNA sequencing (RNA-seq) datasets in response to different environmental stressors can provide clues for identification of condition-specific and/or common AS variants for preferred agronomic traits. In this study, we report five RNA-seq datasets (350.7 Gb) inoculated with one of three bacteria, one virus, or one oomycete. In addition, we obtained existing transcriptome datasets in response to abiotic and biotic stressors, phytohormones, and various developmental stages. From 428 total samples comprising 841.49 Gb, we identified the AS landscape in C. annuum. We further uncovered 1,704,387 AS events through differential AS analysis and identified 6,280 differential alternative splicing (DAS) genes related to environmental stressors, developmental stages, and phytohormones. This information and approach will enable comparative analysis of differential AS from RNA-seq datasets. Additionally, our findings provide useful data for basic-research programs focused on enhancing tolerance to environmental stressors in hot pepper or establishing breeding programs.
Project description:Alternative splicing (AS) is a widely observed phenomenon in eukaryotes that plays a critical role in development and stress responses. In plants, the large number of RNA sequencing (RNA-seq) datasets in response to different environmental stressors can provide clues for identification of condition-specific and/or common AS variants for preferred agronomic traits. In this study, we report five RNA-seq datasets (350.7 Gb) inoculated with one of three bacteria, one virus, or one oomycete. In addition, we obtained existing transcriptome datasets in response to abiotic and biotic stressors, phytohormones, and various developmental stages. From 428 total samples comprising 841.49 Gb, we identified the AS landscape in C. annuum. We further uncovered 1,704,387 AS events through differential AS analysis and identified 6,280 differential alternative splicing (DAS) genes related to environmental stressors, developmental stages, and phytohormones. This information and approach will enable comparative analysis of differential AS from RNA-seq datasets. Additionally, our findings provide useful data for basic-research programs focused on enhancing tolerance to environmental stressors in hot pepper or establishing breeding programs.
Project description:To validate the oligo array and to investigate the impact of the environmental stressors on dolphin health at transcriptomic level Collection of blood samples from wild animals in 4 different geographic locations of the South East of the United States