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Transcriptional profiling of mouse cavenous endothelial cells in normal and high glucose condition

ABSTRACT: Transcriptional profiling of mouse cavenous endothelial cells in normal and high glucose condition

PROVIDER: PRJNA609141 | ENA |

REPOSITORIES: ENA

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Transcriptional profiling of mouse cavenous endothelial cells in normal and high glucose condition

Project description:Purpose: To investigate the potential target genes associated with diabetic condition in mouse cavernous endothelial cells (MCECs) for diabetic induced erectile dysfunction (ED) treatment. Materials and Methods: Mouse cavernous tissue was embedded into Matrigel and sprouted cells were subcultivated for other studies. To mimic diabetic condition, the MCECs were exposed to normal-glucose (NG, 5 mmoL) and high-glucose (HG, 30 mmoL) condition for 72 hours. RNA-sequencing assay was performed to evaluate gene expression profiling and RT-PCR was used for sequencing data validation.

2020-09-01 | GSE146078 | GEO

Genome-wide analysis of normal and high glucose condition

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Genome-wide analysis of normal and high glucose condition

Project description:Genome-wide analysis of normal and high glucose condition

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Genome-wide analysis of High glucose-induced gene expression by DKK2 protein

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Genome-wide analysis of High glucose-induced gene expression by Ninjurin1 siRNA

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HK-2 cell cultured with normal glucose and high glucose

Project description:HK-2 cell cultured with normal glucose and high glucose

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Genome-wide analysis of High glucose-induced gene expression by Ninjurin1 siRNA

Project description:Transcriptional profiling of Mouse cavernous primary endothelial cells (MCECs) in comparing normal glucose condition with high glucose condition or Ninjurin1 siRNA transfection condition. Objective was to determine the effect of ninjurin1 on high glucose conditioned cells on global gene expression. Total RNA obtained from MCECs, which were treated with High glucose (HG) condition or (HG+ninjurin1 siRNA). Biological replicates: 1 control replicates, 1 HG replicates, 1 (HG+ninjurin1 siRNA) replicates.

2014-05-20 | E-GEOD-57525 | biostudies-arrayexpress

Long term high glucose exposure in retinal endothelial cells

Project description:RNA-seq was performed on 4-week high glucose (25mM D-glucose) treated human retinal microvascular endothelial cells (HRMECs) and control (5mM D-glucose) passage-matched cells.

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RNA-seq of young and senescent human umbilical vein endothelial cells (HUVECs) under normal or high glucose conditions

Project description:Human endothelial cellular models are useful to disentangle the pathophysiological role of dysfunctional endothelium in the development of cardiovascular (CV) disease and organ damage in T2D. Here, we performed a RNAseq of human umbilical vein endothelial cells (HUVECs) undergoing replicative senescence and exposed to high glucose (25 mM) to investigate the combined effects of replicative senescence and high glucose on the transcriptional landscape of these cells. To gain insight into the molecular mechanisms driving the acquisition of a senescent phenotype following exposure to HG, we performed a RNA-seq assay on control (Ctr) and replicative senescent (Sen) HUVECs cultivated in presence/absence of HG culture medium (total number of samples = 12; number of samples in each cell type-medium condition group = 3) using the NovaSeq 6000 Illumina system. Differential expression analysis was performed in R environment (version 4.2.2) using the limma and edgeR Bioconductor R packages.

2024-04-08 | E-MTAB-13959 | biostudies-arrayexpress

Transcriptional profiling of glucose deprivation resistant and glucose deprivation sensitive clones derived from IGROV-1 and SKOV3 ovarian cancer models and cultivated under either normal-glucose or low-glucose condition

Project description:This microarray experiment was designed to identify genes and pathways modulated in glucose deprivation resistant (GDR) and glucose deprivation sensitive (GDS) clones of ovarian cancer xenografts. Tumors were established in NOD/SCID mice by s.c. injection of human ovarian cancer cells (IGROV-1 and SKOV3). After sacrifice GDR and GDS clones were obtained from ex vivo cultures of tumors. Once isolated, GDR and GDS clones were cultivated in normal-glucose (0h) or low-glucose condition (6 h and 24 h). Two different time points were selected to investigate both early (6 h) and late (24 h) transcriptional effects of glucose deprivation in IGROV-1 and SKOV3-derived clones. Total RNA was extracted from samples and hybridized on Affymetrix GeneChip™ PrimeView™ Human Gene Expression Arrays. Based on assessment of RNA quality and on quality control analyses (including MAplots and boxplots), only one sample, corresponding to GDR condition at 6 h of glucose deprivation in SKOV3 model, was excluded because it was deemed not suitable for data analysis. In order to evaluate the effects of glucose deprivation in the two models, expression data of IGROV-1 and SKOV3-derived GDR and GDS clones were normalized and analyzed separately. Raw microarray data and pre-processed data matrices are available together with the applied protocols. Results of differential expression analysis are provided as supplementary tables of the associated publication.

2023-05-31 | E-MTAB-12150 | biostudies-arrayexpress

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