Project description:This experiment was performed to study the expression profile of renin cells from mice subjected to homeostatic threat (hypotension). It was also used to compare expression of proliferation associated genes and tumor suppressors with existing cell line data.
Project description:RNA-seq was performed to compare the transcriptome of three renin cell types: juxtaglomerular cells, cells isolated from chronically recruited mice and in As4.1 cells
Project description:We generated genome-wide chromatin-state maps of mouse renin cells: chronically recruited cells and As4.1 cell line. We found that H3K27ac can discriminate a set of regulatory regions unique for the renin cells.
Project description:We have used single-cell RNA-seq to identify transcriptional differences between WT renin cells and renin cells with deletion of the renin gene
Project description:miRNA profiling of mouse kidney arteriolar smooth muscle cells (aSMCs) of the renin lineage comparing control untreated cells with cells treated with forskolin to induce renin expression.
Project description:miRNA profiling of mouse kidney arteriolar smooth muscle cells (aSMCs) of the renin lineage comparing control untreated cells with cells treated with forskolin to induce renin expression. Two condition experiment: control untreated aSMCs vs forskolin treated aSMCs; Biological replicates: control 3, treated 3; independently grown and harvested. One replicate per array.