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RNA sequencing to identify UNC-49-regulated genes in C. elegans defense against pathogen infection

ABSTRACT: RNA sequencing to identify UNC-49-regulated genes in C. elegans defense against pathogen infection

PROVIDER: PRJNA610518 | ENA |

REPOSITORIES: ENA

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RNA sequencing to identify UNC-49-regulated genes in C. elegans defense against pathogen infection

Project description:To gain molecular insights on how UNC-49 regulates C. elegans innate immunity, we used RNA sequencing to profile gene expression in unc-49(e407) animals relative to wild-type animals with or without P. aeruginosa(PA14) infection. We found that UNC-49 suppresses the expression of insulin pathway genes, and lack of UNC-49-mediated suppression in unc-49(e407) animals contributes to their improved survival against P. aeruginosa infection.

2023-10-23 | GSE146406 | GEO

RNA sequencing to identify UNC-25-regulated genes in C. elegans defense against pathogen P. aeruginosa infection

Project description:Purpose: To gain molecular insights on how UNC-25 regulates C. elegans innate immunity, we used RNA sequencing to profile gene expression in unc-25(e156) animals relative to wild-type animals with or without P. aeruginosa (PA14) infection. Methods: Synchronized L1 worms were placed onto NGM plates seeded with E. coli OP50 and grown at 20 °C until the L4 stage. Worms were washed off the plates, collected with M9 solution and subsequently transferred to modified NGM plates containing E. coli OP50 or P. aeruginosa PA14 for 24 h at 25 °C. Then, the animals were washed off the plates with M9 solution and frozen in TRIzol (Transgen, ER501-01, China) with liquid nitrogen. Total RNA was isolated using a TransZol Up Plus RNA kit (Transgen, ER501-01, China). Results: RNA seq analyses shows enriched and signficant changed immune genes, neuropeptides, transcription factors and pathways that are dependent of GABA signaling. Conclusion: Our studies uncover that GABA signaling enhance the resistence to pathogen induced killing.

2023-06-26 | GSE195835 | GEO

RNA-Seq to identify NMUR-1-regulated genes in C. elegans defense against pathogen infection

Project description:To gain molecular insights on how NMUR-1 regulates C. elegans defense against pathogen infection, we used RNA-Seq to profile gene expression in nmur-1(ok1387) animals relative to wild-type animals with or without E. faecalis or S. enterica infection. We found that NMUR-1 modulates C. elegans transcription activity by regulating the expression of transcription factors, which, in turn, controls the expression of distinct immune genes in response to different pathogens.

2022-02-28 | GSE154324 | GEO

RNA sequencing to identify NPR-8-regulated genes in C. elegans defense against pathogen infection

Project description:To gain molecular insights on how NPR-8 regulates C. elegans defense against pathogen infection, we used RNA sequencing to profile gene expression in npr-8(ok1439) animals relative to wild-type animals with or without P. aeruginosa infection. We found that NPR-8 suppresses the expression of genes related to cuticle structure activity including collagen genes, and lack of NPR-8-mediated suppression in npr-8(ok1439) animals contributes to their improved survival against P. aeruginosa infection.

2019-10-15 | GSE122544 | GEO

RNA sequencing to identify immunity-regulated genes in C. elegans defense against pathogen infection

Project description:To understand the molecular mechanism of C. elegans innate immunity, we used RNA sequencing to profile gene expression in wild-type animals with or without P. aeruginosa (PA14) infection. We found that a bZIP transcription factor ZIP-11 is dramatically induced in wild-type worm upon PA14 infection. And ZIP-11 promotes innate immunity through activating PMK-1/p38 pathway and interacting with a CCAAT/enhancer-binding protein CEBP-2.

2022-12-15 | GSE155868 | GEO

ChIP-seq of UNC-30 and UNC-55 in C.elegans

Project description:We performed ChIP-seq for GFP fusion protein of UNC-30 and UNC-55 with endogenous expression

2017-08-28 | GSE102213 | GEO

RNA sequencing to identify NPR-8-regulated genes in C. elegans defense against pathogen infection

Project description:RNA sequencing to identify NPR-8-regulated genes in C. elegans defense against pathogen infection

| PRJNA505517 | ENA

RNA-Seq to identify NMUR-1-regulated genes in C. elegans defense against pathogen infection

Project description:RNA-Seq to identify NMUR-1-regulated genes in C. elegans defense against pathogen infection

| PRJNA645889 | ENA

RNA-seq quantification of expression changes upon unc-62 knockdown in adult Caenorhabditis elegans.

Project description:We performed RNA-seq to quantify gene expression changes in adult worms upon knockdown of transcription factor unc-62/Homothorax. unc-62 is a developmental regulator that binds proximal to age-regulated transcripts and modulates lifespan. In the intestine (in which tissue-specific unc-62 knockdown increases lifespan), we identify multiple effects of unc-62 knockdown linked to extension of longevity. First, unc-62 RNAi decreases the expression of yolk proteins (vitellogenins) that aggregate in the body cavity and become toxic in old age. Second, unc-62 RNAi results in a broad increase in expression of intestinal genes that typically decrease expression with age, suggesting that unc-62 activity balances intestinal resource allocation between yolk protein expression and fertility on the one hand and somatic functions on the other.

2012-09-01 | GSE39574 | GEO

Novosphingobium sp. B3058 49 strain:49

Project description:Novosphingobium sp. B3058 49 strain:49 Genome sequencing

| PRJNA463442 | ENA