Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA editing profile in miRNA loci in human

ABSTRACT: RNA editing profile in miRNA loci in human

PROVIDER: PRJNA612484 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

RNA editing profile in miRNA loci in human [breast]

Project description:RNA editing profile in miRNA loci in human [breast]

| PRJNA511871 | ENA

RNA editing profile in miRNA loci in human [breast]

Project description:We utilized the targeted RNA-seq to identify and quantify RNA editing events located in miRNA and mRNA loci.

2020-04-12 | GSE124374 | GEO

RNA editing profile in miRNA loci in human [ADAR1 KO, Drosha KD]

Project description:We utilized the stranded RNA-seq and small RNA-seq to identify and quantify RNA editing events located in miRNA and mRNA loci.

2020-04-12 | GSE146946 | GEO

RNA editing profile in miRNA loci in human [ADAR1 KO, Drosha KD]

Project description:RNA editing profile in miRNA loci in human [ADAR1 KO, Drosha KD]

| PRJNA612488 | ENA

RNA editing profile in miRNA loci in human

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-04-11 | GSE146947 | GEO

Quantification of RNA editing in the Drosophila Genetic Reference Panel

Project description:We report the application of mmPCR-seq to male whole body samples from 131 strains of the DGRP. We quantified RNA editing at 605 different loci using a microfluidic multiplex PCR method coupled with deep sequencing. RT-PCR amplification of 605 loci from 131 fly strains to quantify RNA editing levels.

2015-08-06 | E-GEOD-67082 | biostudies-arrayexpress

miRNA-independent function of long noncoding pri-miRNA loci

Project description:Among the large, diverse set of mammalian long noncoding RNAs (lncRNAs), long noncoding primary microRNAs (lnc-pri-miRNAs) are those that host miRNAs. Whether lnc-pri-miRNA loci have important biological function independent of their cognate miRNAs is poorly understood. From a genome-scale lncRNA screen, lnc-pri-miRNA loci were enriched for function in cell proliferation, and in glioblastoma (i.e., GBM) cells with DGCR8 or DROSHA knockdown, lnc-pri-miRNA screen hits still regulated cell growth. To molecularly dissect the function of a lnc-pri-miRNA locus, we studied LOC646329 (also known as MIR29HG), which hosts the miR-29a/b1 cluster. In GBM cells, LOC646329 knockdown reduced miR-29a/b1 levels, and these cells exhibited decreased growth. However, genetic deletion of the miR-29a/b1 cluster (LOC646329-miR29Δ) did not decrease cell growth, while knockdown of LOC646329-miR29Δ transcripts reduced cell proliferation. The miR-29a/b1-independent activity of LOC646329 corresponded to enhancer-like activation of a neighboring oncogene (MKLN1), regulating cell propagation. The LOC646329 locus interacts with the MKLN1 promoter, and antisense oligonucleotide knockdown of the lncRNA disrupts these interactions and reduces the enhancer-like activity. More broadly, analysis of genome-wide data from multiple human cell types showed that lnc-pri-miRNA loci are significantly enriched for DNA looping interactions with gene promoters as well as genomic and epigenetic characteristics of transcriptional enhancers. Functional studies of additional lnc-pri-miRNA loci demonstrated cognate miRNA-independent enhancer-like activity. Together, these data demonstrate that lnc-pri-miRNA loci can regulate cell biology via both miRNA-dependent and miRNA-independent mechanisms.

2021-03-12 | GSE137048 | GEO

miRNA-independent function of long noncoding pri-miRNA loci

Project description:miRNA-independent function of long noncoding pri-miRNA loci

| PRJNA564324 | ENA

MicroRNA editing in Xenopus tropicalis

Project description:We collected small RNA sequencing data from brain and heart of an adult Xenopus tropicalis individual to investigate the conservation of site-specific miRNA editing events identified in mammals. Sequencing of 2 small RNA sequencing libraries

2014-06-11 | E-GEOD-56680 | biostudies-arrayexpress

Plant miRNA profile from pooled human serum samples

Project description:Plant miRNA profile from pooled human serum samples

| PRJNA483878 | ENA