Project description:We report expression data analysis comparing HEK 293 and HBEC 5i immortalized cell lines grown for 3 days in serum-free Neurobasal medium
Project description:A validation experiment performed on HEK293 cell lines after genome editing. The design contains three duplicate runs consisted of: HEK293 wild type cell line HEK293 with MIR484 gene knockdown using CRISPR-Cas9 HEK293 with MIR185 gene knockout using CRISPR-Cas9
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived transcriptome profiling of Wild Type and RBM7-/- HEK293 cell lines and RNA immunoprecipitation (RIP) of Flag-tagged RBM7-expressing HEK293 cells
Project description:Auto Immune REgulator (AIRE) protein expression in HEK293 cells Three cell-lines where used in the study: HEK293-NC; control cell-line expressing yellow fluorescent protein (YFP) HEK-AIRE1, HEK-AIRE2; two different clone-derived Autoimmune regulator protein expressing cell-lines HEK-AIRE-D312A; cell line expressing Autoimmune regulator protein with D312A mutation in the first PHD finger With all cell-lines three independent experiments were performed.
Project description:Bulk ATAC-seq performed on whole adult brains across multiple homozigous fly lines (DGRP) in order to find caQTLs. Young adults (1-3 days) were used for all genotypes.