Project description:Transcriptomic profiling and unsupervised clustering of cMyc+ GC-B cells identify the four subpopulations representing positive-selection stages.
Project description:To determine functional overlap between cMyc and AP4 in CD8+ T cell priming, we retrovirally expressed cMyc or AP4 in cMyc-deficient CD8+ T cells and examined gene expression after activation.
Project description:To determine functional overlap between cMyc and AP4 in CD8+ T cell priming, we retrovirally expressed cMyc or AP4 in cMyc-deficient CD8+ T cells and examined gene expression after activation. Naive CD8+ T cells from Myc conditional knockout mice with a tamoxifen inducible Cre transgene were retrovirally transduced with Myc or AP4 followed by a treatment with 4-hydroxytamoxifen in the presence of IL-7 for 2 days. RNA was harvested 48 hours after restimulation of transduced cells with anti-CD3 antibody and gene expression was compared by microarray. CD8+ T cells from littermate wildtype mice that were transduced with an empty retrovirus were used as control.
Project description:Transcriptome profiling of human umbilical vein endothelial cells (HUVECs) transduced with lentiviruses encoding FLAG-CAS9 nuclease and control- (gCTL) or cMYC-targeting (gMYC) gRNAS to identify cMYC regulated genes.
Project description:cMyc is a master regulator of transcription in growing cells. Here, we used microarray to study global transcriptomic expression of cMyc knockdown HT1080 cells to investigate cMyc- regulated gene expression.
