Project description:PUS7, a pseudouridine synthase, plays a critical role in growth, self-renewal, and tumorigenesis of glioblastoma stem cells. We used RNA-seq to investigate the global gene expression regulated by PUS7 and identified downstream pathway of PUS7 in glioblastoma stem cells.
Project description:TLX (NR2E1), as an orphan nuclear receptor, plays a critical role in growth, self-renewal, and tumorigenesis of glioblastoma stem cells. We used microarrays to investigate the global gene expression regulated by TLX and identified downstream targets of TLX in glioblastoma stem cells. We further investigated the global gene expression regulated by TET3, a downstream target of TLX, to identified downstream targets of TLX-TET3 axis in glioblastoma stem cells.
Project description:This project investigates the role METTL8 in human glioblastoma stem cells. In order to understand the transcriptomic changes upon deletion of METTL8, we performed this RNA seq and further downstream analyses to determine the pathways changed. From this, we hope to identify novel methods to target glioblastoma stem cells via manipulation of METTL8.