Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Activating PIK3CA mutation promotes osteogenesis of bone marrow mesenchymal stem cells in macrodactyly

ABSTRACT: Activating PIK3CA mutation promotes osteogenesis of bone marrow mesenchymal stem cells in macrodactyly

PROVIDER: PRJNA621634 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Activating PIK3CA mutation promotes osteogenesis of bone marrow mesenchymal stem cells in macrodactyly

Project description:Comparison of differential expression between macrodactyly BMSC and polydactyly BMSC

2020-07-13 | GSE147823 | GEO

Activating Mutations in PIK3CA result in Widespread Modulation of the Tyrosine Phosphoproteome

Project description:The human oncogene PIK3CA is frequently mutated in human cancers. The two hotspot mutations in PIK3CA, E545K and H1047R, have been shown to regulate widespread signaling events downstream of AKT. However, the impact of these activating mutations on the tyrosine phosphorylation signaling in the cell has not been studied. Here, we performed a global phosphotyrosine profiling using isogenic knockin cell lines containing these activating mutations. We identified 824 unique phosphopeptides from 308 proteins. We found a surprisingly widespread modulation of tyrosine phosphorylation levels of proteins in the knockin mutant cells, with many of the regulated proteins involved in important biological processes, including those in the cytoskeletal migration pathways and kinase regulated signaling. We observed a widespread modulation of the tyrosine kinome, with 24 of the tyrosine kinases showing either upregulation or downregulation in phosphorylation levels. Many of the regulated phosphosites that we identified were located in the kinase domain or the canonical activating sites, indicating that the kinases were active and hence their downstream signaling pathways. Our study thus shows that the activating mutations in PIK3CA result in widespread tyrosine signaling regulation, in addition to the serine/threonine signaling pathways activated by the canonical PI3K-AKT axis.

2018-10-24 | PXD001460 | Pride

Gene expression of human bone marrow mesenchymal stem cell (hBMSCs) during osteogenesis with or without TNF-alpha

Project description:In our previous study, we found that TNF-alpha (100ng/mL) different treatment times have opposing effects on BMSC osteogenesis. To investigate the roles of TNF-alpha in the osteogenic differentiation of hBMSCs, we performed gene microarray to detected the gene expression of hBMSCs with or without TNF-alpha during osteogenesis on days 3 and 4.

2021-06-04 | GSE176086 | GEO

Spinal tuberculosis infection induces osteopenia by reducing the migration and osteogenesis of bone marrow mesenchymal stem cells

Project description:Objectives: Bone marrow mesenchymal stem cells (BMSC) play an important role in osteogenesis. However, the role of BMSC in osteopenia in spinal tuberculosis (STB) patients is unclear. Methods: Bone mineral density (BMD) of 53 STB patients and 55 controls was measured. The migration and osteogenesis of BMSC were assessed by wound healing assays and alizarin red staining, and osteoclast marker expression was measured. RNA-seq was performed on BMSC and the enriched signaling pathways were verified. Results:BMD of STB patients was significantly decreased and BMSC migration and osteogenesis were significantly reduced. After verification, we found that the B-cell receptor signaling pathway, tuberculosis, osteoclast differentiation, Th17-cell differentiation, Ras signaling pathway and PI3K-Akt signaling pathway were affected.And of these pathways, the B-cell receptor signaling, Th17-cell differentiation, Ras signaling pathway and PI3K-Akt signaling pathway were not previously known to be involved in osteopenia. The MAPK signaling pathway and ECM-receptor interactions were correlated with STB prognosis. Conclusion: STB infection can significantly reduce BMSC migration and osteogenesis, decreasing BMD. This process is regulated by multiple pathways and is associated with STB prognosis. Our study suggests that reduced BMSC migration and osteogenesis play important roles in osteopenia in STB patients, and BMSC may be a therapeutic target.

2023-12-01 | GSE222013 | GEO

Structurally-diverse, PPAR?-activating environmental toxicants induce adipogenesis and suppress osteogenesis in bone marrow mesenchymal stromal cells.

Project description: Not available

| S-EPMC4406869 | biostudies-literature

Activating AKT1 and PIK3CA mutations in metastatic castration-resistant prostate cancer

Project description:Activating mutations in AKT1 and PIK3CA are under-characterised in metastatic castration-resistant prostate cancer (mCRPC), but in other cancers are linked to PI3K signaling activation and sensitivity to pathway inhibitors. Our objective was to determine the prevalence, genomic context, and clinical associations of AKT1/PIK3CA activating mutations in mCRPC.

| EGAS00001004328 | EGA

Transcriptome expression of human bone marrow mesenchymal stem cell (hBMSCs) during osteogenesis

Project description:Transcriptome expression of human bone marrow mesenchymal stem cell (hBMSCs) during osteogenesis

| PRJNA740003 | ENA

Expression data of human bone-marrow mesenchymal stem cells treated with ApoA-I for different time-points during osteogenesis

Project description:Human mesenchymal stem cells are differentiated into osteoblasts in the presence or absence of ApoA-I treatment, the global gene profiles of MSCs with different time-points are compared. Day 0 is primary human MSCs without induction was used the baseline for comparision. We used microarrays to investigate the global gene expression profiles in hBM-MSCs with ApoA-I treatment for 1, 3, 5 days during osteogenesis

2018-09-15 | GSE73087 | GEO

Comparison of gingiva-derived and bone marrow mesenchymal stem cells for osteogenesis.

Project description: Not available

| S-EPMC6815943 | biostudies-literature

LIGHT (TNFSF14) enhances osteogenesis of human bone marrow-derived mesenchymal stem cells.

Project description: Not available

| S-EPMC7895395 | biostudies-literature