Project description:We employed single-cell RNA sequencing of islets in young mice subjected to partial pancreatectomy (PPTx). Four clusters of pancreatic beta cells including a subpopulation of replicating beta cells. Pseudo-time course analysis clarified the gradual changes in gene expression from non-replicating beta cells to replicating ones. Upstream analysis visualized the transcriptional networks associated with beta cell replication by PPTx.

2020-11-04 | GSE152730 | GEO

RNA sequencing discerns sub-populations of pancreatic β-cells and dynamics of gene expression in partial pancreatectomy model

2020-11-04 | GSE152731 | GEO

Phytohormone-triggered Transcriptional Reprograming Reveals β-glucosidase as a Key Player for Polysaccharide Metabolism in Dendrobium catenatum

Project description:Dendrobium catenatum (D. catenatum) has long been used as an important medicinal herb in oriental medicine. Polysaccharide, flavonoid, and alkaloid are the major active ingredients, the production and accumulation of which are frequently affected by many environmental factors including water, temperature, and mycorrhizal fungus. However, how phytohormones frequently used in orchid tissue culture regulate the production of active components in D. catenatum is far from clear. Here, major medicinal metabolites were comparatively analyzed in 2-month-old D. catenatum seedlings exposed to phytohormones (0.1 mg/L NAA and/or 1.0 mg/L 6-BA) for three weeks, and eight transcriptomes (two replicates each) corresponding to the treatments were generated. Results demonstrated that the phytohormones triggered transcriptional reprograming, analysis of which revealed a tight association of β-glucosidase (BGLU) expression with the accumulation of polysaccharide. Subsequently, a second RNA-sequencing was further conducted to reveal that a relatively short period (2 w) of varied concentrations of 6-BA application might initially reduce BGLU expression and polysaccharide content and then increase their levels with prolonged treatments (4 w). The RNA-seq data was reconfirmed by functional analysis that 6-BA induced polysaccharide production, while knockdown of BGLU2L resulted in decreased polysaccharide levels. Therefore, the present study clearly demonstrated that BGLU is a key regulator for polysaccharide production and that it is extremely susceptible to NAA and 6-BA treatments in D. catenatum.

2022-05-31 | GSE184090 | GEO

Phytohormone-triggered Transcriptional Reprograming Reveals β-glucosidase as a Key Player for Polysaccharide Metabolism in Dendrobium catenatum

Project description:Phytohormone-triggered Transcriptional Reprograming Reveals β-glucosidase as a Key Player for Polysaccharide Metabolism in Dendrobium catenatum

| PRJNA763165 | ENA

Sphingomonas sp. CDS-1

Project description:Sphingomonas sp. CDS-1 Genome sequencing and assembly

| PRJNA387507 | ENA

Bgl3-a clone encoded Beta-glucosidase from Pectobacterium carotovorum subsp. carotovorum

Project description:Seq [organism=Pectobacterium carotovorum subsp. carotovorum] [clone=Bgl3] gene, Complete cds

| PRJEB42308 | ENA

Project description:sfa D/E partial CDS

| PRJNA555052 | ENA