Project description:Purpose:Comparative cellular and transcriptome analyses was applied to characterize gene expression during male gametophytic development in Brassica carinata. Methods: floral buds (contain two developmental progress,1.1-1.6 mm and 1.8-6.5 long floral buds) were collected, then Separated male organs were kept in liquid nitrogen immediately until use. Total RNA was extracted using the TRIzol reagent (Invitrogen, Waltham, MA, USA). DNase (Promega, USA) was used to remove potential DNA contamination. For the quantitative real-time polymerase chain reaction (qRT-PCR) analysis Results: In this study, Up-regulated expression of DNA methylation probably affected pollen abortion in synthetic allohaploid B. carinata,and Down-regulated expression of cytokinin may affect pollen division and growth in synthetic allohaploid B. carinata Conclusions: Genes were shown male-preferred implies the dynamic changes of DNA methylation during the development of male gametes. The DEGs, related to CK signaling pathway and BR synthesis pathway were highly enriched in developmental male gametes, suggesting that CK played pivotal roles in male gamete development.
