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Transcriptional profiling of mouse germinal center B cells (B220+, GL7+, CD95+) isolated from GC-specific (Cg1-Cre) DAF-TM transgenic or control mice

ABSTRACT: Transcriptional profiling of mouse germinal center B cells (B220+, GL7+, CD95+) isolated from GC-specific (Cg1-Cre) DAF-TM transgenic or control mice

PROVIDER: PRJNA625050 | ENA |

REPOSITORIES: ENA

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Transcriptional profiling of mouse germinal center B cells (B220+, GL7+, CD95+) isolated from GC-specific (Cg1-Cre) DAF-TM transgenic or control mice

Project description:Germinal centers (GCs) support diversification and affinity maturation of antibody repertoires through iterative cycles of T-cell-dependent positive selection, B cell clonal expansion and antigen-receptor hypermutation. Positive selection is critical for efficient affinity maturation and depends on only partially understood signaling processes. We show that BCL6-dependent physiological repression of decay accelerating factor (DAF/CD55) on GC B cells is critical for effective positive selection and affinity maturation during GC responses. Absence of DAF on GC B cells lifts restraint on local complement activation, yielding autocrine C3a/C5a-receptor signaling that is indispensable for CD40-dependent mTOR pathway activation during positive selection. Genetic disruption of this pathway causes premature GC collapse and impairs affinity maturation. These results identify previously undiscerned targets to therapeutically manipulate protective and potentially injurious humoral immune responses.

2021-03-22 | GSE148570 | GEO

Type 1 interferon signaling is crucial from the loss of tolerance nd the development of autoreactive B cells.

Project description:The transcriptomes of FACS-sorted B220+GL7+CD95+ germinal center B cells and B220+GL7-CD95- non-germinal center B cells from lupus-prone B6.Sle1b were compared to the same cell types in B6.Sle1b mice without the type 1 interferon receptor (B6.Sle1b.IFNaR1-/-).

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N2, daf-1(m40), and daf-1(m40);daf-3(mgDf90) adult, gravid hermaphrodites

Project description:total RNA from N2, daf-1(m40), and daf-1(m40);daf-3(mgDf90) adult, gravid hermaphrodites were extracted and sequenced.

2020-05-28 | GSE131997 | GEO

RNAseq of dauer-forming C. elegans L2d in daf-2 and daf-2;daf-42

Project description:Under adverse environmental conditions, nematodes arrest into dauer, an alternative developmental stage for diapause. Dauers endure unfavorable environment and interact with host animals to access favorable environments, thus playing a critical role in the survival of both free-living and parasitic nematodes. Here, we discovered that in Caenorhabditis elegans, daf-42 is essential for development into dauer stage, as the null mutant shows lethal phenotype during dauer entry. To examine the transcriptional changes accompanied by the absence of DAF-42 during dauer entry, we performed RNA-sequencing on daf-2 and daf-2; daf-42 worms at 52 hours after egg laying (HAE) and 60 HAE, the time at which 0% and about 40% of daf-2; daf-42 mutants form dead dauer at 25 degrees Celcius, respectively. daf-2 control worms develop into dauer stage after 60 HAE, and half of its population develop into dauer by 72 HAE, when raised at 25 degrees Celcius.

2023-04-23 | GSE230353 | GEO

Transcription profiling of C. elegans mutants daf-2(e1368) and daf-2(e1368);pmk-1(km25), between daf-2(e1368), daf-2(e1368);daf-16(mgDf47)

Project description:Analysis of genes differentially expressed between daf-2(e1368) and daf-2(e1368);pmk-1(km25) and between daf-2(e1368) and daf-2(e1368);daf-16(mgDf47). These studies identified genes upregulated by wild-type PMK-1 and wild-type DAF-16. Experiment Overall Design: Three independent biological replicates were isolated for each strain. All strains in a replicate were grown and harvested in parallel.

2008-06-14 | E-GEOD-5801 | biostudies-arrayexpress

Transcriptional profiling of WT-ProB, WT-PreB and ProB cells from Rag1ko/ko, IghTer5H/Ter5H, IghTer5 /wt;Rag1ko/ko , IghTer5H∆TM/ Ter5H∆TM and IghTer5H∆TM/ Ter5H∆TM ;Rag1ko/ko mice

Project description:We studied the role of the stable Igh mRNA in Igh chain check point. Here we generated RNA-seq data from sorted ProB (CD19+B220+c-Kit+CD25-) and PreB (CD19+B220+c-Kit-CD25+) populations from Bone marrow of mice.

2020-12-29 | GSE144275 | GEO

Methylobacterium sp. TM 27 strain:TM 27

Project description:Methylobacterium sp. TM 27 strain:TM 27 Genome sequencing

| PRJNA347036 | ENA

Genes regulated by PMK-1 and DAF-16 in a daf-2(e1368) background.

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DAF-16/FoxO and EGL-27/GATA promote developmental growth in response to persistent somatic DNA damage [N2, daf-2, daf-16, daf-2;daf-16]

Project description:Genome maintenance defects cause complex disease phenotypes characterized by developmental failure, cancer susceptibility and premature aging. It remains poorly understood how DNA damage responses function during organismal development and maintain tissue functionality when DNA damage accumulates with aging. Here we show that the FoxO transcription factor DAF-16 is activated in response to DNA damage during development while the DNA damage responsiveness of DAF-16 declines with aging. We find that in contrast to its established role in mediating starvation arrest, DAF-16 alleviates DNA damage induced developmental arrest and even in the absence of DNA repair promotes developmental growth and enhances somatic tissue functionality. We demonstrate that the GATA transcription factor EGL-27 co-regulates DAF-16 target genes in response to DNA damage and together with DAF-16 promotes developmental growth. We propose that EGL-27/GATA activity specifies DAF-16 mediated DNA damage responses to enable developmental progression and to prolong tissue functioning when DNA damage persists.

2014-11-18 | GSE51162 | GEO

C. elegans microarrays: daf-2(-) vs. daf-2(-) xbp-1(-)

Project description:Transcriptional profiling of day 1 animals comparing untreated daf-2(-) animals and daf-2(-) xbp-1(-) animals. Goal was to identify genes whose expression in daf-2 mutants is dependent (directly or indirectly) on xbp-1.

2010-02-03 | GSE20148 | GEO

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