Human lymphoid stromal cell heterogeneity and functional alteration in follicular lymphoma
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ABSTRACT:
PROVIDER: PRJNA625285 | ENA |
REPOSITORIES: ENA
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Project description:Despite recent advances in the characterization of stromal cell origin, heterogeneity, and function in mice, little is known in human. Follicular lymphoma (FL) is the most frequent indolent lymphoma. It corresponds to the accumulation of germinal center B-cell clones within lymph nodes. FL B cells interact with a reprogrammed immune and stromal cells that provide crucial contributions to the licensing of tumor growth and immune evasion. Most of transcriptomics approaches in human was performed on cultured, and amplified stromal cells modifying their phenotype and function. We purified native stromal cells from LN FL patients, healthy tonsil, and healthy bone marrow. Gene expression profiling based on three lymphoid stromal cell types provides new insights on the characterization of those cells in human and highlight new signalling pathways involved in the crosstalk between stromal cells and tumoral FL B cells.
2021-04-30 | GSE148656 | GEO
Project description:Lymph node (LN) stromal cells provide survival signals and adhesive substrata to lymphocytes. During an immune response, B cell follicles enlarge, questioning how LN stromal cells manage these cellular demands. Herein, we used a murine fate mapping system to describe a new stromal cell type that resides in the T cell zone of resting LNs. We used microarrays to detail the global programme of gene expression characterizing this new population of lymphoid stromal cells. Mice lymph nodes were collected and digested before cell sorting and RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of lymphoid stromal cells in order to increase expression profiles.
2013-12-31 | E-GEOD-47906 | biostudies-arrayexpress
Project description:20 FL lymph node samples from 16 patients and four normal lymphoid tissues (two tonsil and two spleen) from four different patient. Data can be viewed on the supplemental website at http://genome-www.stanford.edu/rituximab/. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Using regression correlation
2005-11-22 | E-GEOD-3646 | biostudies-arrayexpress
Project description:Human bone marrow mesenchymal stromal cells (BM-MSC) could be committed toward a functional lymphoid-like stroma by a combination of TNFalpha (TNF) and Lymphotoxin alpha1/beta2 (LT) (Amé-Thomas et al Blood 2007). Bone marrow and lymph node stromal cells support FL malignant cell recruitment and growth in particular after comittment to a lymphoid-like differentiation in vitro. In addition, more than 70% of FL patients exhibit a bone marrow involvment at diagnosis. We delineate using Affymetrix U133+2.0 microarrays the gene expression profile of BM-MSC obtained from FL patients (FL-MSC) and age-matched healthy donors (HD-MSC) in order to identify a specific FL-MSC signature. In addition, we used Affymetrix microarrays to define the gene expression signature of lymphoid-like stromal cells obtained from HD-MSC by treatment with TNF/LT in vitro. This TNF/LT signature was then used to interpret the gene expression profile of FL-MSC. GEP was performed on 10 BM-MSC from FL patients and 6 from healthy donors, treated or not with TNF(10 ng/mL)/LT(100ng/mL)
2012-01-26 | E-GEOD-35331 | biostudies-arrayexpress
Project description:This SuperSeries is composed of the following subset Series: GSE3646: Follicular lymphoma and normal lymphoid tissue comparisons GSE3647: Follicular lymphoma lymph node Abstract: Analysis of the patterns of gene expression in follicular lymphomas from 24 patients suggested that two groups of tumors might be distinguished. All patients, whose biopsies were obtained before any treatment, were treated with rituximab, a monoclonal antibody directed against the B cell antigen, CD20. Gene expression patterns in the tumors that subsequently failed to respond to rituximab appeared more similar to those of normal lymphoid tissues than to gene expression patterns of tumors from rituximab responders. These findings suggest the possibility that the response of follicular lymphoma to rituximab treatment may be predicted from the gene expression pattern of tumors. Refer to individual Series
2006-03-06 | E-GEOD-4351 | biostudies-arrayexpress
Project description:Sequencing Follicular Lymphoma
| PRJNA348784 | ENA
Project description:20 FL lymph node samples from 16 patients and four normal lymphoid tissues (two tonsil and two spleen) from four different patient. Data can be viewed on the supplemental website at http://genome-www.stanford.edu/rituximab/. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set
2005-11-23 | GSE3646 | GEO
Project description:Human bone marrow mesenchymal stromal cells (BM-MSC) could be committed toward a functional lymphoid-like stroma by a combination of TNFalpha (TNF) and Lymphotoxin alpha1/beta2 (LT) (Amé-Thomas et al Blood 2007). Bone marrow and lymph node stromal cells support FL malignant cell recruitment and growth in particular after comittment to a lymphoid-like differentiation in vitro. In addition, more than 70% of FL patients exhibit a bone marrow involvment at diagnosis. We delineate using Affymetrix U133+2.0 microarrays the gene expression profile of BM-MSC obtained from FL patients (FL-MSC) and age-matched healthy donors (HD-MSC) in order to identify a specific FL-MSC signature. In addition, we used Affymetrix microarrays to define the gene expression signature of lymphoid-like stromal cells obtained from HD-MSC by treatment with TNF/LT in vitro. This TNF/LT signature was then used to interpret the gene expression profile of FL-MSC.
2012-01-26 | GSE35331 | GEO
Project description:Lymph node (LN) stromal cells provide survival signals and adhesive substrata to lymphocytes. During an immune response, B cell follicles enlarge, questioning how LN stromal cells manage these cellular demands. Herein, we used a murine fate mapping system to describe a new stromal cell type that resides in the T cell zone of resting LNs. We used microarrays to detail the global programme of gene expression characterizing this new population of lymphoid stromal cells.
2013-12-31 | GSE47906 | GEO
Project description:20 FL lymph node samples from 16 patients and four normal lymphoid tissues (two tonsil and two spleen) from four different patient. Data can be viewed on the supplemental website at http://genome-www.stanford.edu/rituximab/.
2005-10-05 | E-SMDB-1845 | biostudies-arrayexpress