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Cell-intrinsic differences between human tracheal epithelial cells from children and adults [FACS-sorted basal cells]

ABSTRACT: Cell-intrinsic differences between human tracheal epithelial cells from children and adults [FACS-sorted basal cells]

PROVIDER: PRJNA625888 | ENA |

REPOSITORIES: ENA

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Cell-intrinsic differences between human tracheal epithelial cells from children and adults [FACS-sorted basal cells]

Project description:The airway epithelium is a key protective barrier whose integrity is preserved by the self-renewal and differentiation of basal progenitor cells. Epithelial cells are central to the pathogenesis of multiple chronic lung diseases for which age is a principle risk factor. Children are also less susceptible to SARS-CoV-2 infection, suffer less severe symptoms than adults and have a lower rate of mortality. Few studies have addressed differences between airway epithelial cells in children and adults. Here, we perform bulk RNA sequencing studies in laser-captured whole epithelium, FACS-sorted basal cells and cultured basal cells, as well as in vitro cell proliferation experiments, to address the intrinsic molecular differences between paediatric and adult airway basal cells. We find that, while the cellular composition of the paediatric and adult tracheal epithelium is broadly similar, in cell culture, paediatric airway epithelial cells displayed higher colony forming ability, better in vitro growth and outcompeted adult cells in competitive proliferation assays. Although recurring differences between airway epithelial gene expression were seen between samples from children and adults, RNA sequencing showed broad conservation of transcriptional programmes. Genes associated with SARS-CoV-2 infection were not differentially expressed between children and adults, although individuals showed some variability in their expression of viral infection-associated genes. Our results chart important cell intrinsic differences in transcriptional profile and regenerative capacity between tracheal epithelial cells of children and adults.

2020-04-20 | GSE148816 | GEO

Cell-intrinsic differences between human tracheal epithelial cells from children and adults [cultured basal cells]

2020-04-20 | GSE148817 | GEO

Cell-intrinsic differences between human tracheal epithelial cells from children and adults [laser-captured fresh epithelial cells]

2020-04-20 | GSE148815 | GEO

Cell-intrinsic differences between human tracheal epithelial cells from children and adults [cultured basal cells]

Project description:Cell-intrinsic differences between human tracheal epithelial cells from children and adults [cultured basal cells]

| PRJNA625892 | ENA

Cell-intrinsic differences between human tracheal epithelial cells from children and adults

Project description:Cell-intrinsic differences between human tracheal epithelial cells from children and adults

| PRJNA625886 | ENA

Cell-intrinsic differences between human tracheal epithelial cells from children and adults [laser-captured fresh epithelial cells]

Project description:Cell-intrinsic differences between human tracheal epithelial cells from children and adults [laser-captured fresh epithelial cells]

| PRJNA625889 | ENA

Cell-intrinsic differences between human tracheal epithelial cells from children and adults

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-04-20 | GSE148818 | GEO

mTOR Inhibition Enables Long-Term Expansion of Human Neonatal Tracheal Aspirate-Derived Airway Basal Stem Cells

Project description:Bronchopulmonary dysplasia remains one of the most common complication of prematurity, despite significant improvements in perinatal care. Functional modeling of human lung development and disease, like BPD, is limited by our ability to access the lung and to maintain relevant stem cell populations in culture. Single cell RNA-sequencing confirmed the presence of epithelial cells in tracheal aspirates obtained from intubated neonates. Using combined SMAD signaling inhibition and mTOR inhibition neonatal tracheal-aspirate derived (nTAD) basal stem cells can be expanded long-term and retain the ability to differentiate into pseudo-stratified airway epithelium. Conclusions: Our data demonstrate that neonatal tracheal aspirate-derived epithelial cells can provide a novel ex vivo human cellular model to study neonatal lung development and disease.

2020-04-15 | GSE137353 | GEO

mTOR Inhibition Enables Long-Term Expansion of Human Neonatal Tracheal Aspirate-Derived Airway Basal Stem Cells

2020-09-21 | GSE137950 | GEO

The Ciliated Cell Transcriptome: Ciliated Mouse Tracheal Epithelial Cells vs Control Non-ciliated Mouse Tracheal Epithelial Cells during in Vitro Differentiation

Project description:Transcriptional profiling of ciliating mouse tracheal epithelial cells compared to non-ciliating cells at two timepoints, ALI+4 and ALI+12, during differentiation in vitro. These cells are obtained from a transgenic mouse expressing GFP from a human FOXJ1 promoter, so that cells destined to become ciliated can be sorted by FACS based on their expression of GFP. Likewise, the control non-ciliated cells are identified by their lack of GFP expression. Ciliated cells were compared to Universal Reference RNA, and non-ciliated cells were compared to Universal Reference RNA.

2012-11-29 | GSE42500 | GEO

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