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ZEB2-mediated EMT in CRC cell line DLD-1

ABSTRACT: ZEB2-mediated EMT in CRC cell line DLD-1

PROVIDER: PRJNA625909 | ENA |

REPOSITORIES: ENA

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ZEB2-mediated EMT in CRC cell line DLD-1

Project description:We report the induction of epithelial-mesenchymal transition (EMT) in the epithelial colorectal cancer (CRC) cell line DLD-1. ZEB2 expression is sufficient to induce the downregulation of epithelial markers such as E-cadherin, claudins, keratins and occludins, while upregulating the expression of several mesenchymal markers as vimentin and fibronectin.

2020-04-18 | GSE148823 | GEO

Identification of ZEB2 binding sites in Hepatocellular Carcinoma Cell Line SNU398

Project description:ZEB2, a member of the ZEB family of EMT inducers, is involved in cellular proliferation, senescence, and invasion in most of the human cancers. However, genes regulated by ZEB2 in hepatocellular carcinoma (HCC) are not identified so far. Hence, we conducted a ChIP-Seq study in high ZEB2 expressing HCC cell line SNU398 by using a homemade anti-ZEB2 antibody (clone 6E5).

2018-03-08 | GSE103048 | GEO

Mass spectrometry analysis for IP with NEIL1 Antibody in CRC DLD-1 cell

Project description:NEIL1 overexpression was performed in CRC DLD-1 cells. The protein interacted with NEIL1 were analyzed by Mass spectrometry. The NEIL1 overexpressed and control cells in the logarithmic growth phase were collected for IP with NEIL1 Antibody.

2023-03-15 | PXD040878 | iProX

ZEB2 protects the heart from ischemic damage

Project description:infarct size and subsequent deterioration in function. The identification of factors that enhance cardiac repair by the restoration of the vascular network is, therefore, of great significance. Here, we show that the transcription factor Zinc finger E-box-binding homeobox 2 (ZEB2) is increased in stressed cardiomyocytes and induces a cardioprotective cross-talk between cardiomyocytes and endothelial cells to enhance angiogenesis after ischemia. Single-cell sequencing indicates ZEB2 to be enriched in injured cardiomyocytes. Cardiomyocyte-specific deletion of ZEB2 results in impaired cardiac contractility and infarct healing post-myocardial infarction (post-MI), while cardiomyocyte-specific ZEB2 overexpression improves cardiomyocyte survival and cardiac function. We identified Thymosin 4 (TMSB4) and Prothymosin (PTMA) as main paracrine factors released from cardiomyocytes to stimulate angiogenesis by enhancing endothelial cell migration, and whose regulation is validated in our in vivo models. Therapeutic delivery of ZEB2 to cardiomyocytes in the infarcted heart induces the expression of TMSB4 and PTMA, which enhances angiogenesis and prevents cardiac dysfunction. These findings reveal ZEB2 as a beneficial factor during ischemic injury, which may hold promise for the identification of new therapies.

2021-03-23 | PXD022212 | Pride

Differential gene expression between DLD-1 cells lines with moderately and significantly upregulated levels of Snail

Project description:Using human colon cancer DLD-1 cells, we engineered a cell line with a doxycycline-inducible single-copy of Snail and compared it to an existing EMT models in DLD-1 where Snail was introduced by episome transfection. Induction of the single-copy line (Snail-lo) with doxycycline increased Snail expression to a level similar to that observed in cancer cell lines spontaneously expressing Snail and results in partial EMT. In comparison, higher levels of overexpression arising from introduction of episomal-Snail (Snail-hi), results in complete EMT.

2022-09-03 | GSE212490 | GEO

RNA-sequencing in MDA-231-D cells transfected with ZEB1 or ZEB2 siRNAs

Project description:We searched for roles of ZEB1and/or ZEB2 during EMT by RNA-seq in breast cancer cells.

2017-06-16 | GSE98273 | GEO

Expression data for HMLE-based EMT models

Project description:EMT was induced using stable overexpression of 1 of 4 EMT transcription factors (FOXQ1, TWIST1, ZEB2, and SNAI1) in the HMLE cell line. HMLE cells with ectopic LACZ expression were used as control cells.

2021-08-03 | GSE181322 | GEO

ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation

Project description:Comparison of genes associated with the EMT between trophoblast cell line (BeWo, JEG3) controls and cells overexpressing the ZEB2 gene (BeWo ZEB2oe, JEG_ZEB2oe).

2018-10-01 | GSE116532 | GEO

Comparison of expression data from DLD-1 subpopulations III

Project description:The function of cell-cell contact for radiochemosensitivity is unclear. Here, we investigate the role of the E-cadherin/catenin complex proteins under more physiological three-dimensional (3D) cell culture conditions in a panel of CRC cell lines. To gain further insights, differential gene expression was investigated in DLD-1 subpopulations showing distinct morphology and invasion in 3D collagen type I.

2018-03-14 | GSE109046 | GEO

Comparison of expression data from DLD-1 subpopulations II

2018-03-14 | GSE109045 | GEO

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