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Limited Cell Fluorescence Activated Cell-sort Sequencing (LC-FACSeq) is a method for detecting rare clones in leukemia

ABSTRACT: Limited Cell Fluorescence Activated Cell-sort Sequencing (LC-FACSeq) is a method for detecting rare clones in leukemia

PROVIDER: PRJNA629044 | ENA |

REPOSITORIES: ENA

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Limited Cell Fluorescence Activated Cell-sort Sequencing (LC-FACSeq) is a method for detecting rare clones in leukemia

Project description:Detecting and monitoring tumor clonal evolution in the setting of measurable residual disease is technically difficult due to 1) high sequencing background due to limited numbers of disease cells, 2) loss of tumor genetic material due to the need for high quality purified DNA, and 3) resource and bioinformatics intensive requirements of traditional next generation sequencing techniques. Limited Cell Fluorescence Activated Cell-sort Sequencing (LC-FACSeq), is a fast, reproducible, and cost effective amplicon based sequencing technique that does not require genomic material isolation. It can be used in the context of just a few hundred tumor cells and is easily generalization for monitoring clonal evolution in various different malignancies.

2020-08-10 | GSE149545 | GEO

LC-FACSeq is a method for detecting rare clones in leukemia.

Project description: Not available

| S-EPMC7406301 | biostudies-literature

Chromatium okenii LC strain:LC

Project description:Chromatium okenii LC strain:LC Genome sequencing

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Lactobacillus phage Lc-Nu

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Sequencing of ponatinib-resistant LC-2/ad derivatives (PR1 and PR2) and parental LC-2/ad cells

Project description:Sequencing of ponatinib-resistant LC-2/ad derivatives (PR1 and PR2) and parental LC-2/ad cells was performed in order to determine mechanisms of acquired resistance in the PR1 and PR2 cell lines. NRAS p.Q61K mutation identified in PR1 cells. Upregulation of wild-type EGFR signaling identified in PR2 cells.

2017-04-21 | GSE98030 | GEO

Cerebellum of Lurcher (Lc +/-) mouse.

Project description:Analysis of gene expression in cerebellum of postnatal day 10 Lurcher (Lc +/-) mouse, compared to wild type littermate. Keywords: other

2003-03-24 | GSE352 | GEO

Cerebellum of Lurcher (Lc +/-) mouse.

Project description:Analysis of gene expression in cerebellum of postnatal day 10 Lurcher (Lc +/-) mouse, compared to wild type littermate. Keywords: other

2003-03-23 | E-GEOD-352 | biostudies-arrayexpress

Chlamydia trachomatis D-LC

Project description:Chlamydia trachomatis D-LC genome sequencing

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Effects of optogenetic and chemogenetic LC activation on the hippocampal transcriptome

Project description:The locus coeruleus noradrenergic (LC-NA) system plays an important role in organizing a physiological response to acute stress. However, if and how the LC affects stress-mediated transcriptomic changes in the brain is still barely understood. Here, we extensively characterize LC mediated transcriptomic response during acute stress in the hippocampus. Combining for the first time bulk mRNA-sequencing and selective chemogenetic and optogenetic manipulations of the LC-NA system

2023-02-02 | GSE218313 | GEO

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