Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiling in murine mononuclear phagocyte lineage cells

ABSTRACT: Gene expression profiling in murine mononuclear phagocyte lineage cells

PROVIDER: PRJNA630057 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Murine mononuclear phagocyte lineage cells

Project description:Murine mononuclear phagocyte lineage cells

| PRJNA630056 | ENA

Gene expression profiling in murine mononuclear phagocyte lineage cells

Project description:To understand the mechanism underlying monocyte and dendritic cell development through the regulation of Irf8 expression by the 56 kb downstream (+56 kb) Irf8 enhancer, we performed transcriptome analysis of bone marrow cells and splenocytes from wild-type, the Irf8 +56 kb enhancer-deficient, and IRF8-deficient mice. Taken together with the epigenetic profiling of mononuclear phagocyte lineage cells in these mice, the Irf8 +56 kb enhancer-mediated high Irf8 expression in hematopoietic progenitor cells promote type 1 classical dendritic cell (cDC1) differentiation, while low Irf8 expression in progenitors led to Ly6C+ monocyte development.

2020-12-20 | GSE149761 | GEO

Genome wide profiling of chromatin accessibilities in murine mononuclear phagocyte lineage progenitor cells

Project description:Genome wide profiling of chromatin accessibilities in murine mononuclear phagocyte lineage progenitor cells

| PRJNA629970 | ENA

Genome wide profiling of chromatin accessibilities in murine mononuclear phagocyte lineage progenitor cells

Project description:To understand the mechanism underlying monocyte and dendritic cell development through the regulation of Irf8 expression by the 56 kb downstream (+56 kb) Irf8 enhancer, we analyzed chromatin accessibility of bone marrow cells from wild-type and Irf8-/- mice. Combined with the epigenetic profiling by H3K27 acetylation ChIP-seq, the +56 kb enhancer opens at LMPP stage, and gets activated at its highest level in mononuclear phagocyte lineage progenitor cells.

2020-12-20 | GSE149756 | GEO

Single-cell RNA profiling of mononuclear phagocyte in colorectal cancer

Project description:During mononuclear phagocyte system evolution, monocytes differentiate into pro-tumorigenic TAMs. We used single cell RNA sequencing (scRNA-seq) to analyze the characteristics of mononuclear phagocyte in colorectal cancer

2023-07-01 | GSE217774 | GEO

Genome wide profiling of active enhancers and IRF8-binding sites in murine mononuclear phagocyte lineage cells

Project description:To understand the mechanism underlying monocyte and dendritic cell development through the regulation of Irf8 expression by the 56 kb downstream (+56 kb) Irf8 enhancer, we performed epigenetic profiling of bone marrow cells and splenocytes from wild-type, the Irf8 +56 kb enhancer-deficient, and IRF8-deficient mice. Taken together with the transcriptome analysis of mononuclear phagocyte lineage cells in these mice, the Irf8 +56 kb enhancer-mediated high Irf8 expression in hematopoietic progenitor cells promote type 1 classical dendritic cell (cDC1) differentiation, while low Irf8 expression in progenitors led to Ly6C+ monocyte development. In addition, IRF8 ChIP-seq of mature cDC1s and monocytes suggested that IRF8 regulates enhancers in cooperation with different transcription factors in each lineage in its expression level.

2020-12-20 | GSE149757 | GEO

Genome wide profiling of active enhancers and IRF8-binding sites in murine mononuclear phagocyte lineage cells

Project description:Genome wide profiling of active enhancers and IRF8-binding sites in murine mononuclear phagocyte lineage cells

| PRJNA629971 | ENA

Mononuclear phagocyte phenotypes in a multiple sclerosis model

Project description:Mononuclear phagocytes are key regulators of both tissue damage and repair in neuroinflammatory conditions such as multiple sclerosis (MS). To examine divergent phagocyte phenotypes in the inflamed central nervous system (CNS) we introduce an in vivo imaging approach combined with RNAseq and proteomics that allows us to temporally and spatially resolve the evolution of phagocyte polarization in a murine MS model. We show that the initial pro-inflammatory polarization of phagocytes is established after spinal cord entry and critically depends on the compartment they enter. Guided by signals from the CNS environment individual phagocytes then switch their phenotype as lesions move from expansion to resolution. Our study thus provides a first real-time analysis of the temporo-spatial determinants and regulatory principles of phagocyte specification in the inflamed CNS.

2019-07-10 | PXD010245 | Pride

Gene expression data from Peyer's patch mononuclear phagocyte system

Project description:PeyerM-bM-^@M-^Ys patches (PP) are primary inductive sites of mucosal immunity. Defining PP mononuclear phagocyte system (MPS) is thus crucial to understand the initiation of mucosal immune response. We provide a comprehensive analysis of the phenotype, distribution, ontogeny, lifespan, function and transcriptional profile of PP MPS. We show that monocytes give rise to macrophages and to lysozyme-expressing DC (LysoDC) which are both involved in particulate antigen uptake, display strong innate antiviral and antibacterial gene signatures and, upon TLR7 stimulation, secrete IL-6 and TNF but no IL-10. However, unlike macrophages, LysoDC display a rapid renewal rate, strongly express genes of the MHCII presentation pathway and prime naM-CM-/ve helper T cells for IFNg production. Our results show that in PP, at steady state, monocytes generate both LysoDC and macrophages which display distinct features from their adjacent villus counterparts. 3 replicates of 3 different mononuclear phagocyte subsets have been extracted from Peyer's Patches of WT C57Bl/6 mice: CD11b+ DC, lysoDC and lysoMac. The total RNA of PP-sorted cells from the 3 independent experiments was extracted with a Qiagen micro RNAeasy PLUS kit. Quantity, quality and absence of genomic DNA contamination were assessed with a Bioanalyser (Agilent). Microarray experiments were performed by the Plateforme Biopuces of Strasbourg (France) using the GeneChipM-BM-. Mouse Gene 1.0 ST array.

2015-02-03 | E-GEOD-65514 | biostudies-arrayexpress

CSF1R defines the Mononuclear Phagocyte System lineage in human blood in health and disease

Project description:CSF1R defines the Mononuclear Phagocyte System lineage in human blood in health and disease

| PRJNA679277 | ENA