Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Neuronal Activity Increases Translocator Protein (TSPO) Levels in the Adult Mouse Brain

ABSTRACT: Neuronal Activity Increases Translocator Protein (TSPO) Levels in the Adult Mouse Brain

PROVIDER: PRJNA632602 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Neuronal Activity Increases Translocator Protein (TSPO) Levels in the Adult Mouse Brain

Project description:Background: The mitochondrial protein, translocator protein (TSPO), is a widely used biomarker of neuroinflammation, but its non-selective cellular expression pattern implies roles beyond inflammatory processes. The present study investigated whether neuronal activity modifies TSPO expression in the adult central nervous system. Methods: Single-cell RNA sequencing was performed to generate a cellular landscape of basal TSPO gene expression in the hippocampus of adult (12 weeks old) C57BL6/N mice, whereas confocal laser scanning microscopy was used to verify TSPO protein in neuronal and non-neuronal cell populations. TSPO RNA and protein were quantified after stimulating neuronal activity with distinct stimuli, including designer receptors exclusively activated by designer drugs (DREADDs), exposure to a novel environment and acute treatment with the psychostimulant drug, amphetamine. Results: Single-cell RNA sequencing demonstrated a non-selective and multi-cellular gene expression pattern of TSPO at basal conditions in the adult mouse hippocampus. Confocal laser scanning microscopy confirmed that TSPO protein is expressed in neuronal and non-neuronal (astrocytes, microglia, vascular endothelial cells) cells of cortical (medial prefrontal cortex) and subcortical (hippocampus) brain regions. Stimulating neuronal activity through chemogenetic (DREADDs), physiological (novel environment exposure) or psychopharmacological (amphetamine treatment) approaches led to consistent increases in TSPO gene and protein levels in neurons but not in microglia or astrocytes. Conclusions: Neuronal activity has the potential to modify TSPO expression in the adult central nervous system. These findings challenge the general assumption that altered TSPO levels unequivocally mirrors ongoing neuroinflammation and emphasize the need to consider non-inflammatory interpretations in some physiological or pathological contexts.

2022-05-01 | GSE150508 | GEO

Neuronal activity increases translocator protein (TSPO) levels.

Project description: Not available

| S-EPMC8440208 | biostudies-literature

Brain neuronal cell nuclei with exceptionally high levels of hydroxymethylcytosine

Project description:We recently reported that a significant subpopulation of adult mouse brain cellular nuclei (NeuN-High) have highly decondensed chromatin characteristic of multipotent cells types (Yu et al., DNEU, 2015). Herein we show that NeuN-High nuclei also have significantly higher levels of 5´-hydroxymethylcytosine (5hmC), a rate limiting intermediate in the turnover of modified cytosine. TET-assisted bisulfite sequencing demonstrated that 5hmC levels in NeuN-High nuclei spiked rapidly from 20% to nearly 40% of CG dinucleotides at the start of gene regions for various classes of the most highly transcribed neuron-specific genes, but were variably distributed among different functional gene categories and in well-defined gene regions. NeuN-Low neuronal and non-nueronal neuronal nuclei had significantly lower levels of 5hmC for all classes of expressed genes. In summary, these data support the view that an elevated turnover rate for chromatin modifications may potentiate the cellular memory of the most active subclasses of neurons.

2022-07-07 | GSE72432 | GEO

Neuronal activity modifies the chromatin accessibility landscape in the adult brain (RNA-Seq)

Project description:We generated RNA profiling datasets from adult mouse dentate gyrus upon neuronal activation at three different time point

2017-01-08 | GSE86366 | GEO

Neuronal activity modifies the chromatin accessibility landscape in the adult brain (ATAC-Seq)

Project description:We generated the chromatin accessibility profiling datasets from adult mouse dentate gyrus upon neuronal activation at 3 different time points

2017-01-08 | GSE86365 | GEO

Neuronal activity modifies the chromatin accessibility landscape in the adult brain (RNA-Seq WT)

Project description:We generated RNA profiling datasets from adult mouse dentate gyrus upon neuronal activation at three different time point

2017-01-08 | GSE82013 | GEO

Neuronal activity modifies the chromatin accessibility landscape in the adult brain (RNA-Seq OE)

Project description:We generated RNA profiling datasets from adult mouse dentate gyrus with cFos knockdown and/or overexpression upon neuronal activation

2017-01-08 | GSE82014 | GEO

Neuronal activity modifies the chromatin accessibility landscape in the adult brain (ATAC-Seq WT)

Project description:We generated the chromatin accessibility profiling datasets from adult mouse dentate gyrus upon neuronal activation at 3 different time points

2017-01-08 | GSE82010 | GEO

Neuronal activity modifies the chromatin accessibility landscape in the adult brain (ATAC-Seq KD)

Project description:We generated the chromatin accessibility profiling datasets from adult mouse dentate gyrus with cFos knockdown upon neuronal activation (E0 and E1)

2017-01-08 | GSE82012 | GEO

Neuronal activity modifies the chromatin accessibility landscape in the adult brain (ATAC-Seq OE)

Project description:We generated the chromatin accessibility profiling datasets from adult mouse dentate gyrus with cFos knockdown and overexpression upon neuronal activation (E0 and E1)

2017-01-08 | GSE82011 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data