Project description:The aim of the study is to identify KMT9α target genes in AOM/DSS tumour organoids, APK and APKK organoids, patient derived CRC organoids, AOM/DSS mouse tumours and normal mouse colon

2021-11-29 | GSE150503 | GEO

KMT9 controls growth and stemness of colorectal cancer [ChIP-seq]

Project description:The aim of the study is to identify KMT9a target genes in AOMDSS tumour organoids

2021-11-29 | GSE150502 | GEO

KMT9 controls growth and stemness of colorectal cancer [single-cell RNA-seq]

Project description:The aim of the study is to identify cell-specific KMT9a target genes in AOMDSS tumour organoids

2021-11-29 | GSE150505 | GEO

ZFP90 promotes colorectal carcinogenesis and stemness

Project description:ZFP90 promotes colorectal carcinogenesis and stemness

| PRJNA497880 | ENA

KMT9 is an actionable target in muscle-invasive bladder cancer

Project description:Novel treatment modalities are imperative for the challenging management of muscle-invasive and metastatic BC to improve patient survival rates. The recently identified lysine methyltransferase (KMT) 9, an obligate heterodimer composed of KMT9α and KMT9β, regulates the growth of various types of tumors such as prostate, lung and colon cancer. While overexpression of KMT9α was previously observed to be associated with aggressive basal-like MIBC in an analysis of patients’ tissue samples, a potential functional role of KMT9 in this type of cancer has not been investigated to date. In this study, we show that KMT9 regulates proliferation and migration of various MIBC cell lines with different genetic mutations. KMT9α depletion results in differential expression of genes involved in the regulation of the cell cycle, cell adhesion and migration. Reduced cell proliferation upon KMT9α depletion is also observed in Pten/Trp53 knockout bladder tumor organoids, which cannot be rescued with an enzymatically inactive KMT9α mutant. In accordance with the idea that the catalytic activity of KMT9 is required for the control of cellular processes in MIBC, a recently developed small molecule inhibitor of KMT9 (KMI169) also impairs cancer cell proliferation. Since KMT9α depletion also restricts the growth of xenografts in mice, our data suggest that KMT9 is an actionable novel therapeutic target for the treatment of MIBC.

2024-05-01 | GSE255344 | GEO

FIBP maintains colorectal cancer cells stemness via demethylation of stemness genes

Project description:FIBP maintains colorectal cancer cells stemness via demethylation of stemness genes

| PRJNA412214 | ENA