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Assessment of transcriptional ERa activity following exposure to 17a-E2 and 17b-E2

ABSTRACT: Assessment of transcriptional ERa activity following exposure to 17a-E2 and 17b-E2

PROVIDER: PRJNA634479 | ENA |

REPOSITORIES: ENA

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Assessment of transcriptional ERa activity following exposure to 17a-E2 and 17b-E2

Project description:The study shows that, regardless of dose, both estrogens elicit nearly identical ERa activity

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Project description:To further study gene expression regulated by 17b-estradiol in uteri, we have employed whole gene microarray profiling to identify genes specifically regulated by the Estrogen receptor ERÎ± localized either at the plasma membrane or in the nucleus. Two mice models have been used, the mice named ERa-AF2Â° (named AF2_KO and on C57BL6/J background) in which 7 aminoacids have been deleted in the AF2 domain and the mice C451A-ERa (named MISS_KO and on C57BL6/N background) mutated for the C451 into Ala on ERa. Control littermates were used for each mutant mice respectively name AF2_WT or MISS_WT. 17b-estradiol induced gene expression in uteri from ovariectomized mice was measured after 6 hours exposure to 0 (PLB) and 8Âµg/kg of 17b-estradiol (E2) injected subcutaneously in castor oil.

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Estradiol Induced Transcriptional Profile of EAAE DNA Binding Deficient ERa Uterus

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Project description:Cutibacterium acnes 17B Genome sequencing

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