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Driving Intracellular Metal Ions of Human Induced Pluripotent Stem Cells with Intense Terahertz Pulses

ABSTRACT: Driving Intracellular Metal Ions of Human Induced Pluripotent Stem Cells with Intense Terahertz Pulses

PROVIDER: PRJNA636346 | ENA |

REPOSITORIES: ENA

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Driving Intracellular Metal Ions of Human Induced Pluripotent Stem Cells with Intense Terahertz Pulses

Project description:Terahertz (THz) radiation has been exploited for biomedical applications for non-invasive manipulation of living cells. Here we describe an apparatus to apply high-field THz excitation on highly environmental sensitive human induced pluripotent stem cells (hiPSCs), and manipulate the gene expression networks associated with zinc-finger (ZNF) transcription factors.

2020-06-02 | GSE151549 | GEO

A novel peptide hydrogel of metal ion clusters for accelerating bone defect regeneration

Project description:We explore the genes and pathways related to metal ions involved in extracellular matrix, cell adhension and migration, expression of cytokine, antibacterial, inflammation, and cell signaling.

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RNAseq of human epithelial lung carcinoma cell line A549 exposed to ceria, silver or zinc ions, micron-sized particles or nanoparticles

Project description:The toxicity of silver and zinc oxide nanoparticles is hypothesised to be mediated by dissolved metal ions and cerium dioxide nanoparticles (CeO2 NPs) are hypothesised to induce toxicity specifically by oxidative stress dependant on their surface redox state. To test these hypotheses, RNAseq was applied to characterise the molecular responses of cells to metal nanoparticle and metal ion exposures. The human epithelial lung carcinoma cell line A549 was exposed to different CeO2 NPs with different surface charges, micron-sized and nano-sized silver particles and silver ions, micron-sized and nano-sized zinc oxide particles and zinc ions, or control conditions, for 1 hour, 6 hours and 24 hours. Concentrations were the lower of either EC20 or 128 micrograms/mL. Transcriptional responses were characterised by RNAseq transcriptomics using an Illumina HiSeq2500 .

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Intense THz pulses down-regulate genes associated with skin cancer and psoriasis: a new therapeutic avenue?

Project description:We show that exposure of artificial human skin tissue to intense, picosecond-duration THz pulses affects expression levels of numerous genes associated with non-melanoma skin cancers, psoriasis and atopic dermatitis. Genes affected by intense THz pulses include nearly half of the epidermal differentiation complex (EDC) members. EDC genes, which are mapped to the chromosomal human region 1q21, encode for proteins that partake in epidermal differentiation and are often overexpressed in conditions such as psoriasis and skin cancer. In nearly all the genes differentially expressed by exposure to intense THz pulses, the induced changes in transcription levels are opposite to disease-related changes.

2013-07-09 | GSE48586 | GEO

Intense THz pulses down-regulate genes associated with skin cancer and psoriasis: a new therapeutic avenue?

2013-07-09 | E-GEOD-48586 | biostudies-arrayexpress

Infection of macrophages with functional Slc11a1 yields differences in global gene expression, regulation of metal ions, and infection outcomes

Project description:Manipulation of zinc at the host-pathogen interface depends on both the identity of the pathogen and the nature of the host cell. In this study, we examine the response of bone marrow derived macrophages from 129S6/SvEvTac mice to infection by Salmonella Typhimurium. Unlike Balb/c and C57BL/6 mice, 129S6/SvEvTac mice possess a functional Slc11a1 (Nramp-1), a phagosomal transporter of divalent cations. We examine the changes in gene expression upon treatment with live or heat killed Salmonella at 2 Hrs and 18 Hrs post infection, and observed widespread changes in metal transport, metal-dependent, and metal homeostasis genes, suggesting significant remodeling of iron, copper, and zinc availability by host cells.

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Next Generation Sequencing Facilitates Quantitative Analysis of Acinetobacter baylyi ADP1 Transcriptomes under heavy metal-based NPs and ions exposure

Project description:The goals of this study are to use Next-generation sequencing (NGS)to detect bacterial mRNA profiles of Acinetobacter baylyi ADP1 in response to 0, 1 mg/L AgNPs or Ag ions, 1 mg/L CuO NPs or Cu ions, 10 mg/L ZnO NPs or Zn ions for 2 h, in triplicate, using Illumina HiSeq 2500.The NGS QC toolkit (version 2.3.3) was used to treat the raw sequence reads to trim the 3’-end residual adaptors and primers, and the ambiguous characters in the reads were removed. Then, the sequence reads consisting of at least 85% bases were progressively trimmed at the 3’-ends until a quality value ≥ 20 were kept. Downstream analyses were performed using the generated clean reads of no shorter than 75 bp. The clean reads of each sample were aligned to the E. coli reference genome (NC_000913) using SeqAlto (version 0.5). Cufflinks (version 2.2.1) was used to calculate the strand-specific coverage for each gene, and to analyze the differential expression in triplicate bacterial cell cultures. The statistical analyses and visualization were conducted using CummeRbund package in R (http://compbio.mit.edu/cummeRbund/). Gene expression was calculated as fragments per kilobase of a gene per million mapped reads (FPKM, a normalized value generated from the frequency of detection and the length of a given gene.

2020-10-23 | GSE139295 | GEO

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Project description:METAL RUST Metagenome

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Infection of macrophages with functional Slc11a1 yields differences in global gene expression, regulation of metal ions, and infection outcomes

Project description:Infection of macrophages with functional Slc11a1 yields differences in global gene expression, regulation of metal ions, and infection outcomes

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Next Generation Sequencing Facilitates Quantitative Analysis of Acinetobacter baylyi ADP1 Transcriptomes under heavy metal-based NPs and ions exposure

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Acinetobacter baylyi ADP1 Transcriptomes under heavy metal-based NPs and ions exposure

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