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Analysis of the coding and non-coding RNA temporal transcriptome of developing seeds in sunflower

ABSTRACT: Analysis of the coding and non-coding RNA temporal transcriptome of developing seeds in sunflower

PROVIDER: PRJNA637232 | ENA |

REPOSITORIES: ENA

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Analysis of the coding and non-coding RNA temporal transcriptome of developing seeds in sunflower [RNA-seq]

Project description:Analysis of the coding and non-coding RNA temporal transcriptome of developing seeds in sunflower [RNA-seq]

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Analysis of the coding and non-coding RNA temporal transcriptome of developing seeds in sunflower [ncRNA-seq]

Project description:Analysis of the coding and non-coding RNA temporal transcriptome of developing seeds in sunflower [ncRNA-seq]

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Analysis of the coding and non-coding RNA temporal transcriptome of developing seeds in sunflower [ncRNA-seq]

Project description:miRNA-sequencing of seeds in three developmental stages was performed to reveals gene regulation in relation to oil and fatty acid accumulation in sunflower

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Analysis of the coding and non-coding RNA temporal transcriptome of developing seeds in sunflower [RNA-seq]

Project description:RNA-sequencing (lncRNA,mRNA and circRNA) of seeds in three developmental stages was performed to reveals gene regulation in relation to oil and fatty acid accumulation in sunflower

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Genome-wide mapping of histone H3 Lysine 4 trimethylation (H3K4me3) and its Involvement in fatty acid biosynthesis in sunflower developing seeds.

Project description:Histone modifications are of paramount importance during plant development. Investigating chromatin remodeling in developing oilseeds sheds light on the molecular mechanisms controlling fatty acid metabolism as well as facilitates the identification of new functional regions in oil crop genomes. The present study characterized the epigenetic modifications H3K4me3 in relationship with the expression of fatty acid related-genes and transcription factors in developing sunflower seeds.

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affy_sunflower_2010_13-ROS and hormones in sunflower seed dormancy.

Project description:affy_sunflower_2010_13 - affy_sunflower_2010_13 - It concerns the interaction between ROS and hormones in dormancy release in sunflower seeds. ABA is responsible for dormancy maintenance, while GA and ethylene promote seed germination. Based on our results, ROS could represent good candidate to shift from a hormone signalling to another determining the dormancy state in sunflower seeds.-We aim to understand the mechanisms controlling sunflower seed dormancy at the transcriptomic level, by the application of treatments which maintain dormancy as ABA, or alleviate dormancy as ROS and ethylene. Transcripts comparison will be performed between dormant and non-dormant sunflower embryo imbibed 24h on water, on ABA, on methylviologen, a pro-oxidant compound or on ethylene.

2013-08-01 | GSE44165 | GEO

Analysis of the coding and non-coding RNA temporal transcriptome of developing seeds in sunflower

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-06-05 | GSE151779 | GEO

affy_sunflower_2010_13-ROS and hormones in sunflower seed dormancy.

2013-08-01 | E-GEOD-44165 | biostudies-arrayexpress

affy_tour_2012-02-Study of sunflower seed dormancy by translatome analysis

Project description:affy_tour_2012-02 - Identification of transcripts that are addressed to traduction in imbibed seeds in relation with dormancy: comparison of the translatome in Dormant versus Non-Dormant seeds -- At harvest seeds are dormant. They stay dormant if they are stored at -20°C (D) and become non-dormant (ND) if they are stored 2 months at +20°C. Polysomal fractions were purified on sucrose gradients from sunflower axis isolated from dormant and non-dormant seeds imbibed at 10°C during 3h, 15h or 24h. - These fractions allow to identify the transcripts addressed to translation (translatome) during the seed imbibition process (3, 15 and 24h) - The translatome of 2 types of seeds are compared: Dormant vs Non-Dormant at the 3 time points. 18 arrays - SUNFLOWER; time course,treated vs untreated comparison

2013-10-30 | E-GEOD-41944 | biostudies-arrayexpress

RNASeq of Developing Arabidopsis Seeds Producing Hydroxy-Fatty Acids

Project description:Arabidopsis seeds expressing the castor fatty acid hydroxylase accumulate hydroxylated fatty acids up to 17% of total fatty acids in seed triacylglycerols, however total seed oil is also reduced up to 50%. Investigations into the cause of the reduced oil phenotype through in vivo [14C]acteate and [3H]2O metabolic labeling of developing seeds surprisingly revealed that the rate of de novo fatty acid synthesis within the transgenic seeds was approximately half that of control seeds. Addition of castor phospholipid:diacylglycerol acyltransferase (PDAT) increased hydroxylated fatty acid content of the seed oil, increased the rate of fatty acid synthesis, and mostly restored seed oil levels. RNAseq analysis indicated no changes in expression of fatty acid synthesis genes in hydroxylase-expressing plants. Transcript profiles of Arabidopsis developing seeds of three lines, at three stages of development were generated by deep sequencing, in triplicate, using Illumina.

2014-01-10 | E-GEOD-53952 | biostudies-arrayexpress

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