Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single Cell Sequencing Reveals Artifactual Gene Expression Following Dissociation in All CNS Cells

ABSTRACT: Single Cell Sequencing Reveals Artifactual Gene Expression Following Dissociation in All CNS Cells

PROVIDER: PRJNA638597 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Single Cell Sequencing Reveals Artifactual Gene Expression Following Dissociation in All CNS Cells

Project description:Single Cell Sequencing of all CNS cell types via enzymatic digestion with or without inhibitor cocktail present.

2020-12-01 | GSE152182 | GEO

Single Cell Sequencing Reveals Artifactual Gene Expression in Microglia in Response to Tissue Dissociation

Project description:Single Cell Sequencing Reveals Artifactual Gene Expression in Microglia in Response to Tissue Dissociation

| PRJNA638598 | ENA

Single Cell Sequencing Reveals Artifactual Gene Expression in Microglia in Response to Tissue Dissociation

Project description:Single Cell Sequencing of FACS purified microglia/myeloid cells isolated with different dissociation protocols

2020-12-01 | GSE152183 | GEO

Antimicrobial immunity impedes CNS vascular repair following brain injury

Project description:Antimicrobial immunity impedes CNS vascular repair following brain injury

| PRJNA722016 | ENA

Single-cell sequencing reveals dissociation-induced gene expression in tissue subpopulations

Project description:Single-cell sequencing reveals dissociation-induced gene expression in tissue subpopulations

| PRJNA339266 | ENA

Single Nucleus Sequencing Reveals Artifactual Gene Expression and Variance in Post-Mortem Brain

Project description:Single Nucleus Sequencing Reveals Artifactual Gene Expression and Variance in Post-Mortem Brain

| PRJNA662657 | ENA

ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Project description:ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

| PRJNA631686 | ENA

ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Project description:ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

| PRJNA702503 | ENA

Single Cell Sequencing Reveals Artifactual Gene Expression in Microglia in Response to Experimental Variability

Project description:Single Cell Sequencing Reveals Artifactual Gene Expression in Microglia in Response to Experimental Variability

| PRJNA638641 | ENA

Single-cell sequencing reveals dissociation-induced gene expression in tissue subpopulations

Project description:In many gene expression studies, cells are extracted by tissue dissociation and Fluorescence-Activated Cell Sorting (FACS), but the effect of these protocols on cellular transcriptomes is not well characterized and often ignored. Here, we applied single-cell mRNA sequencing (scRNA-seq) to muscle stem cells, and unexpectedly found a subpopulation that is strongly affected by the widely-used dissociation protocol that we employed. One implication of this finding is that several published transcriptomics studies may need to be reinterpreted. Importantly, we detected similar subpopulations in other single-cell datasets, suggesting that cells from other tissues might be affected by this artefact as well.

2017-09-28 | GSE85755 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data